Serum Caspase-3,Caspase-9 Activity In Lubar Intervertebral Disc Protrusion Patients

Objective:To explore the relationship between apoptosis promoting effector molecules caspase-3,caspase-9 and lubar intervertebral disc protrusion.Methods:99 of operation patients with lubar intervertebral disc protrusion were recruited.Among them,single segment of lubar intervertebral disc protrusion were 70(Group A),more than one segments of lubar intervertebral disc protrusion were 29(Group B).In addition,40 unrelated healthy people from physical examination center were enrolled as controls(Group C).Enzyme-linked immunosorbent assay(ELISA)was used to examine serum caspase-3,caspase-9 levels in lubar intervertebral disc protrusion patients.Results:The level of caspase-3 in Group A,Group B,Group C respectively were 14.31 ± 0.67,17.43 ±1.86,11.24 ± 0.41 pmol/L.The caspase-3 activity in each group was statistically significant difference(F=8.47,P<0.01).Compared with Group C,the caspase-3 activity in Group B(q=5.78,P<0.01),Group A(q=3.08,P=0.014)was statistically significant difference.The caspase-3 activity in lubar intervertebral disc protrusion patients subgroups(Group A,Group B)was statistically significant difference(q=3.21,P=0.025).The level of caspase-9 in Group A,Group B,Group C respectively were 30.57±3.63,43.68±5.15,18.54±2.19/pmol/L.The caspase-9 activity in each group was statistically significant difference(F=7.85,P=0.001).Compared with Group C,the caspase-9 activity in Group B(q=4.50,P<0.01),Group A(q=3.29,P=0.022)was statistically significant difference.The caspase-9 activity in lubar intervertebral disc protrusion patients subgroups(Group A,Group B)was statistically significant difference(q=3.22,P=0.025).Age between each groups was not statistically significant difference(F=0.091,P=0.91).Male/female ratio between each groups was not statistically significant difference(X2=0.229,P=0.S92).Conclusion:The augment of caspase-3,caspase-9 promotes apoptosis of lubar intervertebral disc protrusion.It is connected with quantity of protrusive segments.The more segments of protrusion,the higher caspase-3,caspase-9 levels of examination will be.