Our country is snort of water,p ana k resources,so it is of great significance to study the technology of saving water and fertilizer in agriculture.The research on promoting the absorption of water and mineral nutrition,saving water and fertilizer,improving quality and increasing yield by making use of organic nutrients or biological stimulants produced by beneficial microbial fermentation agents has been increasingly recognized and valued by people.Plant-derived enzymes made from plant materials with low degree of lignification and fibrosis and fermented by beneficial microorganisms with indigenous advantages contain a variety of nutrients,growth stimulating substances and growth regulating substances required for plant growth,which can promote plant growth,improve quality and improve disease resistance.In order to provide a reference for the development and application of humulus scandens enzyme,this test compared with 100%mineral nutrients and water respectively,studied the different treatment effect on the growth,yield and quality of lettuce by replacing the mineral nutrient solution to make root treatment and spraying on the leaf with different dilution multiple enzyme liquid under the condition of hydroponic lettuce has been accomplished.The main research results are as follows:Humulus scandens enzyme can promote the growth and improve the nutritional quality of lettuce.Compared with the control group,the dry weight,fresh weight and the content of soluble protein,soluble sugar,anthocyanin and Vc of lettuce respectively increased by 2.16%,4.51%,19.95%,38.82%,7.4%and 63.64%,when the humulus enzyme diluted 50 times substituted with 25%mineral nutrient solution by volume ratio;The plant height,stem diameter,maximum root length,dry weight and fresh weight of lettuce was 3.59%,5.18%,31.63%,17.78%,12.37%higher respectively,besides the soluble sugar content,Vc content,soluble protein content and anthocyanin content were increased by 13.64%,21.21%,42.67%,78.57%respectively,when the Humulus scandens enzyme diluted 300 times used as a foliar application of foliar fertilizer.Either replacing the mineral nutrient solution to make root treatment or spraying on the leaf with Humulus scandens enzyme,The activities of antioxidant enzymes and the contents of mineral nutrients,chlorophyll and carotenoids in leaf were all increased.Compared with the control,SOD activity was 74.69%and 89.98%higher respectively,POD enzyme activity increased by 10.82%and 23.61%respectively,CAT enzyme activity was increased by 27.54%and 24.11%respectively,when the humulus enzyme diluted 50 times and 100 times substituted with 25%mineral nutrient solution by volume ratio;Humulus scandens enzyme diluted 300 times sprayed on the leaf has the best effect,it can significantly increase the calcium content of lettuce,furthermore,the activities of SOD and CAT enzymes in the leaf of lettuce were increased by 89.78%and 50%respectively,and the contents of chlorophyll and carotenoid were increased by 25.71%and 33.33%respectively.The light and performance of lettuce can be improved,either the Humulus scandens enzyme replacing the mineral nutrient solution to make root treatment or spraying on the leaf.The effect of the treatment that hunulus enzyme diluted 300 times substituted with 25%mineral nutrient solution by volume ratio is the most significant.Compared with the control,the net photosynthetic rate,the intercellular carbon dioxide concentration and the transpiration rate of the lettuce that after planted twenty days significantly increased by 44.25%,14.13%and 38.68%respectively,When humulus scandens was diluted by 50 times or 200 times,the promotion extent of their relative indexes were lower than those when humulus scandens was diluted by 300 times.Moreover,the time for the difference of the maximum improvement amplitude was also delayed by 15 days and 10 days respectively.The leaf sprayed with humulus enzyme that diluted by 300 times can significantly increase the net photosynthetic rate,stomatal conductance and intercellular carbon dioxide concentration of lettuce,significantly reduce the transpiration rate of lettuce,and the relevant indexes reached the maximum value at 28 days after spraying.To sum up,the humulus enzyme can promote the growth,quality,activity of antioxidant enzyme,accumulation of mineral elements and improvement of photosynthetic performance of lettuce,either replacing the mineral nutrient solution to make root treatment or spraying on the leaf with it.Otherwise,the effect on increasing yield and improving quality by humulus enzyme diluted 50 times substituted with 25%mineral nutrient solution by volume ratio to make root treatment and Humulus scandens enzyme diluted 300 times sprayed on the leaf was both better.So they both can be used in production. |