Study On The Light Condition And Transcriptome Analysis During The Primordial Differentiation Of Pleurotus Eryngii

Pleurotus eryngii belongs to a fungus with high value of edible,nutrition and health care.In recent years,the development of P.eryngii industries in China are rapidly,in order to stabilize industrial production technology,improve production and quality,basic research is becoming more and more important.The quality of primordium differentiation directly affects the growth of fruiting body,and the light condition is the key environmental factor affecting primordium differentiation.At present,there are few studies on the effect of light conditions during the primordium differentiation period of P.eryngii.In this paper,the primordium differentiation process of P.eryngii was studied by red light,yellow light,green light,blue light,white light and darkness.The time of hyphal kink,the time of primordium differentiation and the number of mushroom buds were observed and recorded.At the same time,the agronomic characters of fruiting body,the activity of extracellular enzymes(Carboxymethyl cellulase,hemicellulase,amylase)and the contents of trehalose and cAMP were determined.On this basis,we further analyzed the transcriptome sequencing of primordial samples under hyphae,dark and white light conditions,in order to explore the metabolic characteristics of differential expression during primordium differentiation and the differential genes related to primordium differentiation.It lays a foundation for further study on physiology and biochemistry of P.eryngii during primordium differentiation and provides scientific basis for scientific cultivation of P.eryngii.The main results are as follows:(1)The primordium differentiation of P.eryngii needs the induction and stimulation of light,and it is difficult to differentiate under dark condition.(2)There were significant differences in solid morphology and yield among different light treatments.The biological efficiency of white light treatment group was the highest,the stalk of green light treatment group was the longest and the fresh weight of single mushroom was the largest,and there was no significant difference in commodity rate between white light and green photonic entity.These results show that green light can be used as a reference light quality in production.(3)The change trend of extracellular enzyme activity in cultivation substrate under different light quality treatment was basically similar,and the change trend of hemicellulase activity was the same as that of Carboxymethyl cellulase.Amylase activity showed an upward trend in the process of primordium differentiation.(4)The content of cAMP was increasing during the growth and development of P.eryngii,which indicated that there was a certain relationship between cAMP and the growth development of P.eryngii,but the effect of light quality was not obvious.(5)The trehalose content in the mycelium of red light,green light,white light and dark treatment showed a downward trend.The trehalose content decreased continuously in the early stage of hyphae kink,then increased,and then decreased after the primordium formation,and the overall change showed an S-shaped trend,and the decreasing trend of trehalose content in the green light treatment group was more obvious than that in the white light treatment group.It is speculated that trehalose in the mycelium irradiated by green light is easier to transfer to the primordium.(6)The results of transcriptome analysis showed that MAPK pathway(MAPK signaling pathway),Hedgehog signaling pathway,ABC transporter(ABC transporters),cytochrome P450(cytochrome P450),TGF-? signaling pathway(TGF-beta signaling pathway);).Notch signaling pathway(Notch signaling pathway)and other metabolic pathways are more active during primordial differentiation.Inference are related to the differentiation of primordia.