| Pleurotus eryngii (DC. Ex. Fr.) Quel is a kind of edible, pharmaceutical and hygienical rare edible fungi which has the fastest development in past few years. In this study,34 P. eryngii were collected for analysis and evaluation by using antagonistic action, RAPD, ISSR, SSR molecular markers, esterase isozyme profiles and agronomic characters test. The results obtained in the present study were as follows:1.Antagonistic action showed that there was no antagonistic line between strains 121402 and 131403, the two strains may come from the same parent. Besides, antagonistic lines were existed in the remaining 32 strains, which belonged to different P. eryngii varieties.2.In RAPD molecular makers,266 bands were amplified with 22 primers, each of which could amplify 12.1 bands averagely. In the all bands,217 ones were polymorphic, and the polymorphism rate was 81.6%.34 P. eryngii strains were devided into five groups in 0.75 similarity level. Strains B0703 and B2501 were divided into 2 classes, respectively, which had further relationships with others. The coefficient is 0.95 between 121402 and 131403, which may come from the same parent.3.ISSR analysis showed that 23 primers amplified 212 bands, of which 184 ones were polymorphic. The polymorphism rate was up to 81.6%. Averagely 9.2 bands were exsited in each primer.34 P. eryngii strains were devided into four groups in 0.74 similarity degree. B0303 and B0304 had a closer relationship. B0703 belonged to one chass lonely. The coefficient is 0.93 between 121402 and 131403.4.In SSR molecular makers,103 bands were amplified with 16 primer pairs, each of which could amplify 6.4 bands averagely. In the all bands,83 ones were polymorphic, and the polymorphism rate was 78.9%.34 P. eryngii strains were devided into five groups in 0.75 similarity level. The strains B0703 and B2501 were divided into 2 classes, respectively. The coefficient is 0.93 between 121402 and 131403.5.Esterase isozyme profiles test indicated that 34 P. eryngii strains were devided into six classes in 0.75 similarity degree. The first group contained 17 strains such as B0101, B1601 and so on. The second group included 3 strains B1301, B2501 and B2001. The third group included 9 strains such as B0206 and B0601, etc. Six strains such as B0303, B0304 and so on belonged to the fourth class. B0703 and B2401 were the last two chasses, respectively. B2101 and 131403 had a 100% similarity, the same as B1402 and B3001.6.Agronomic characters test contained full bag rate in 28th and 29th day, growth rate, yield each bag, biological conversion rate, fruiting body number, shape, stipe diameter, cap characters and diameter, Stipe diameter and length.34 P. eryngii strains were devided into six classes According to the above analysis. The first group contained 9 strains such as B0206, B0501, B0601, B1501, B1202, B1801,121402 and 131403, etc. The second group included 2 strains B0601 and B0904. The third group included 8 strains such as B0101, B0401, B1701, B2701, B2901, B3001, B1102 and B3101. Seven strains such as B2001, B2101, B2201, B2501, B2801, B2301 and B2401 belonged to the fourth class. B0303 and B0304 belonged to the fifth class. B1901 was the sixth chass. In addition, B0703, B1301, B1402, B1601 and B2601 were not classified because no nomal fruiting body was generated on them.RAPD and ISSR molecular markers analysis had better application of evaluation. Next well methods were antagonistic action, SSR molecular markers and agronomic characters test. The results of esterase isozyme profiles were not ideal. SSR markers successfully applied in the assessment of P. eryngii resources for the first time. This study played a boost to the development of edible fungi genetic diversity, and it provided the data and information reference for genetic breeding work of P. eryngii in the future. |
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