| In order to observe functional additives NO.1 in pregnant sows,effect on production performance and immune function of this study is to choose the health of the great white sow 30,according to the principle of tire nearly divided into experimental group and control group,each group of 15 head,the experimental group feed the pregnancy sow to add 0.15%of the diet,functional additives NO.1 based diet,control the pregnancy sow feed test feeding period of seven days,is feeding period of 60 days.The experimental observation period was from the start of sow breeding to the end of childbirth,and the number of litter,live litter,weak litter,mummy,stillbirth and birth weight were recorded,and the survival rate was calculated as no difference.10 sows in each group were selected for anterior vena cava blood collection at 60 gestation and 1 week before the birth.The antibody levels of foot-mouth disease,pseudorabies and swine fever of 20 sows in the 2 groups were measured respectively,and the physiological and biochemical indexes of progesterone,blood routine,urea nitrogen and creatinine were measured at the same time.The number of offspring,the number of live offspring,birth weight and survival rate were statistically analyzed.SPSS21.0 statistical software was used for one-way anova analysis,and Duncan s method was used for multiple comparisons.In the experimental group,the average number of foetus was 10.20,the average number of live foetus was 9.70,the average survival rate was 95.04%,and the average birth weight was 1.53kg.In the control group,the average number of foetus was 9.90,the average number of live foetus was 8.00,the average survival rate was 82.75%,and the average birth weight was 1.50kg.Compared with the control group,the number of offspring in the experimental group was increased by 3.03%(P>0.05),with no significant difference;the number of live offspring was increased by 21.25%(P<0.05),with significant difference;the survival rate was increased by 14.85%(P<0.05),with significant difference;and the birth weight was increased by 2.00%(P>0.05),with no significant difference.The antibody levels of foot-and-mouth disease,pseudorabies and swine fever in serum of sows were determined and analyzed.The antibody levels of foot-and-mouth disease and swine fever in pregnant sows were determined by direct indirect hemagglutination,and the antibody levels of pseudorabies were determined by enzyme-linked immunoassay.After 60 days of gestation,90%,90%and 100%antibody qualification rates of sows in the experimental group were respectively 90%,90%and 100%,and 60%,70%and 70%antibody qualification rates in the control group were respectively,and 30%,20%and 30%higher in the experimental group than in the control group.One week before the birth,the antibody qualification rates of sows in the experimental group were 100%,90%and 100%respectively,and those in the control group were 80%,80%and 80%respectively.The antibody qualification rates in the experimental group were 20%,10%and 20%higher than those in the control group.The mean antibody qualification rate of sows in the experimental group was 95%,90%and 100%,respectively.The mean antibody qualification rate of sows in the control group was 70%,75%and 75%,respectively.The experimental group was 25%,15%and 25%higher than the control group.The blood routine items and serum biochemical indexes of sows were determined and analyzed.Serum progesterone in sows was determined by abbott I1000R automatic chemiluminescence assay.The mean progesterone levels were 30.45?g/L to the sows in the experimental group and 32.17?g/L to the sows in the control group at the 60th day of gestation.One week before the birth,the average progesterone levels were changed to 9.00?g/L in the experimental group and 10.73?g/L in the control group.Blood routine indexes were determined by Sysmex k-1000 blood cell analyzer.At 60 days of gestation,the mean number of white blood cells in sows in the experimental group was 17.53 X 109/L,and that in the control group was 15.47 X 109/L,which was 13.32%higher than that in the control group(P<0.05),showing a significant difference.The mean red blood cell number of sows in the experimental group was 5.51 X 1012/L,and that of the control group was 5.38 X 1012/L.The experimental group was 2.42%higher than the control group(P>0.05),with no significant difference.The mean erythrocyte distribution width variation coefficient of sows in the experimental group was 15.46%,and that of the control group was 16.41%.The experimental group was 5.79%lower than the control group(P>0.05),with no significant difference.The mean hemoglobin content of sows in the experimental group was 103.48g/L,and that in the control group was 103.37 g/L,which was 0.11%higher than that in the control group(P>0.05),with no significant difference.One week before the birth,the average number of white blood cells in sows in the experimental group was 14.42 X 109/L,while that in the control group was 12.50 X 109/L.The number of white blood cells in the experimental group was 15.36%higher than that in the control group(P<0.05),showing a significant difference.The mean erythrocyte number of sows in the experimental group was 4.55 X 1012/L,and that of the control group was 3.73 X 1012/L,which was 21.98%higher than that of the control group(P<0.05),showing a significant difference.The variation coefficient of mean erythrocyte distribution width of sows in the experimental group was 15.12%,and that in the control group was 17.38%.The experimental group was 13.00%lower than the control group(P<0.05),showing a significant difference.The mean hemoglobin content of sows in the experimental group was 85.53g/L,and that in the control group was 84.07g/L,which was 1.50%higher than that in the control group(P>0.05),with no significant difference.Although the differences of other indexes are not significant,they all have different degrees of optimization.Hitachi 7670 automatic biochemical analyzer was used to determine the composition of urea nitrogen and creatinine.At 60 days of gestation,the average urea nitrogen content in sows in the experimental group was 3.99mmol/L,and that in the control group was 5.01mmol/L.The experimental group was 20.36%lower than that in the control group(P<0.05),showing a significant difference.The mean creatinine content of sows in the experimental group was 197.32?mol/L,while that in the control group was 199.97?mol/L.One week before the birth,the average urea nitrogen content of sows in the experimental group was 3.25mmol/L,while that of the control group was 4.88mmol/L.The experimental group was 33.40%lower than that of the control group(P<0.05),showing a significant difference.The mean creatinine content of sows in the experimental group was 203.71?mol/L,the control group was 205.03?mol/L,the experimental group was 0.64%lower than the control group(P>0.05),the difference was not significant.Conclusion:Adding functional additive NO.1 into the diet can improve the number of live sows and the survival rate of sows.Functional additive NO.1 can enhance the immune effect of sows against foot-and-mouth disease,pseudorabies and swine fever vaccines.Functional additive NO.1 can significantly increase the number of white blood cells and red blood cells in sows,indicating that it can improve the immune level of sows.The clinical recommended dosage is 1.5kg/T feed. |
PDF Full Text Request