| The objective of this experiment is to study the influences of the addition of some recipes of Chinese herbal medicines (CHMs) to the weaning piglets' diet on their growth performances and immune functions .These CHMs, including Astragalus sp., Rhizoma atractyladis, Macrocephaae, Saposhnikovia divericata, Acanthopanax senticosus, Artemisia capillaries,Licorice root, Achyrenthes bitentrata, Houttuynia cordata, Gynostemma pentaphyllum and Radix isatidis were especially selected and formulated into four recipes namely A, B, C, D. All the four recipes of CHMs were prepared by using multifunction-distillation machine in order to obtain the efficacious and bioactive substances , which were finally condensed and dried to become dry powder. This dry powder products were then mixed with some starch and flavour-enhencers and processed into feed additives. The main functional component, astragalocide supposed existing in the products were measured using ultraviolet visible spectrophotometer and the results showed that the contents of astragalocide in recipe A and recipe C were 0.28mg/ml and 0.18mg/ml respectively..The optimal dosage of additives was screened by a series of feeding trials, in which 360 weaned piglets weighing about 7.5kg were used, and each feeding trial used 15 piglets as a group.. Each recipe had three dosages applied: i.e. higher dosage (8%, 1), moderate dosage (0.4%, 2) and lower dosage (0.2%, 3). The results showed that the daily gains, levels of complements (C3, C4) and immunoglobulins ( IgA, IgM and IgG) in A-1 group were significantly higher than those in A-2and A-3 (P<0.05). As regards to the recipe B, C and D, the additions at moderate dosage had higher average daily gains compared to those at higher and lower ones, but all these tested groups had no significant differences in terms of feed conversion rates (FCR).Another feeding trial was conducted to look at the influences of these recipes at optimal dosages on the growth performances and immunological functions of weaning piglets. 225 weighing about 6kg 3-way crossbred piglets were used and divided into 5 groups.. A1,B2,C2 and D2 were used as test groups, while the other group as a control, in which antibiotic additives ( colistin and tylosine) weresubstituted for CHMs. There were 3 repeats for each group. At the 50th day(the. end) of feeding trial, blood samples were collected and a number of immunological parameters including serum IgA, IgM, IgG, total protein (TP), albumin (ALB), globulin (GBL), IgA, IgM, IgG, complements (C3, C4), interlukin-2 (IL-2), y-interferon (r-IFN), ANAE positive rates of T-cell and the subgroups of T-cell CD3, CD4, and CD8 were determined The results showed as follows:l.As compared, to the control, there were no significant differences in respects of daily gain, the incidence of diarrhea, serum C3, C4, TP, ALB, GLB, IgA, IgM, IgG, y-INF, IL-2 and the subgroups of T-cell (CD3, CD4, and CD8) in A-1 group, but the difference in CD4/CD8 ratio was very significant (P<0.01).2.As compared to the control, the average daily gain increased by 6.1% (P<0.05), while the incidence of diarrhea decreased by 37.2% (P<0.05) and the levels of IgA, IgM, IgG, y-INF and IL-2 were raised by 15% ( P<0.05), 11.1% (P<0.05), 13% (P<0.05), 5.5% (P<0.05) and 4.4% (P<0.05), respectively, but the CD4/CD8 ratio was significant different (P<0.01) in B-2group. There were no significant differences ( P>.05) in respects of complements (C3, C4), ANAE positive rate of T-cell, TP, ALB, GLB, CD3, CD4, and CD8.3.As compared to the control, the average daily gain increased by 14.3% (P<0.05), while the incidence of diarrhea decreased by 61.6% (P<0.05) and C3, C4, TP, ALB increased by 16%, 40%, 10.08% (P>.05) and 10.42% (P>0.05) respectively in C-2 group, but there were no significant differences in respects of ANAE positive rate of T-cell, GLB, y-IFN, IL-2, CD3, CD4 and CD8 (P<0.05).4.As compared to the control, the average daily gain increased significantly, while the incidence of diarrhea and mortality reduced markedly , and CD4/CD8 ratio, se... |
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