Research On Tissue Culture Of Lilium Brownii F.E Brown Var.virdulum Baker And Lycium Barbarrum L.and Analysis Of Their Carbohydrate And Protein Components

Objectives:To establish a complete tissue culture system for regeneration of Lilium brownii F.E Brown var.virdulum Baker.Using tender stems of Lycium ruthenicum to find the main factors of the tissue culture of Lycium barbarum L.and improve the existing regenerative tissue system.TO research the nutritional value of Lycium ruthenicum.Methods:In this research,we used the bulblet,tender leaf,flower org an and renewable bulblet of Lilium as explants,which were culture in MS medium provided with many hormones,for example,6-BA,NAA,We had man ged to establish the Lilium system by using the technology of callus and adventitious bud sinduction and researching the majors factors,pa rticularly in explants materials,medium components and training metho ds which effected the induction of callus and adventitious buds.Rese arching three stages of Lycium barbarum L's tissue culture to find t he majors factors of the tissue culture of Lycium barbarum L.and est ablish a complete tissue culture system by means of using tender stem s of Lycium barbarum.The carbohydrate content of cultivated and wild lycium barbarumr was determined by anthracene-concentrated sulfuric a cid method and compared with each other.The protein content of wild a nd cultivated lycium barbarum plants were determined by bovine serum protein and compared with each other.Results:1.The best inducement explants of Lilium brownii F.E Brown var.virdulum Baker are scale leaf and the best disinfection method is 20 s with 70% concentration of alcohol and 10 min with 0.1% concentration of Hgcl.The best medium for primary is MS+0.5mg/L6-BA+0.2mg/LNAA and MS+0.8mg/L6-BA+0.3mg/LNAA in subculture medium is very efficiency.When in the rooting medium 1/2MS+0.5mg/LNAA is the best choice.The best inducement explants of Ning xia Lycium barbarum are stems.The best medium for primary is MS+0.5mg/LNAA+1.0mg/L6-BA and the best rooting medium is1/2MS+NAA0.6mg/L+6-BA1.5mg/L.By comparison,it was found that the content of sugar and protein in the cultivated Chinese Lycium barbarum was superior to that of wild species,which proved that it had higher nutritive value.