Establishment Of Tissue Culture And Suspension Culture Systems For Underground Bulbs Of Lilium Lancifolium In Tibet

The present study was carried out to establish a plant regeneration system of Lilium lancifolium and underground bulbs were selected as the explants. On one hand, the budding spots was induced from explants and then transferred into rooting media to induce plantlets. On the other hand, the explants was transferred onto media to induce calli. Results of the study would provide fundamental supports to induction, domestication and culture of seedlings at a factorial scale, and to studies on medicinal ingredients of Lilium lancifolium.Results show that the best formula for primary culture of the bulbs was MS+ NAA1.5mg/L+BA0.5mg/L, that for regenerating culture was MS+NAA2.0 mg/L+ BA1.0 mg/L, and that for rooting culture was MS + IBA1.0 mg/L + AC 1.0 g/L. Upon transplantation of the in vitro plant seedlings, the survival rate in vermiculites was 93.3%, much higher than that transplanted in sandy soils.It is found that the best formula for callus induced from the bulbs was MS+BA 1.5 mg/L+NAA 0.5 mg/L. The best formula for callus multiplication was MS+BA 1.0 mg/L + NAA 0.5 mg/L. At the same time, the results indicated that dark condition was favorable to the growth of calli and change the content of sugar in the medium had obvious influence on its growth. Supplemented with active carbon of 0.5 g/L can prevent serious brown. Rates of bud formation from well-grown calli was the highest in medium with a formula of MS + NAA1.5 mg/L+BA0.1 mg/L. Yields of growth of suspension culture cells were the highest with no serious brown when medium was MS +BA0.8mg/L+NAA0.3mg/L, adding sucrose of 20 g/L, pH5.8-6.2 was better than in the original medium.