Analysis Of Stress Tolerance Of SlNAC6 Transgenic Arabidopsis And Its Mechanisms

Salt,drought,freezing and other stress factors will directly or indirectly destroy the life processes such as plant growth and reproduction.At the same time,when plants are confronted with adversity stress,in order to better survive,plants will form various regulatory mechanisms that are adaptive and resistant to adversity stress.In these adaptation and regulation processes,transcription factor regulation is particularly important.Among them,NAC transcription factor is a kind of plant-specific transcription factor associated with stress,which can regulate plant growth and development under adverse conditions.In order to study the stress resistance mechanism of transgenic SlNAC6 Arabidopsis thaliana,the transgenic S1NAC6 homozygous Arabidopsis line(L1,L2)obtained earlier in the laboratory was used as the experimental material,and the PBI121-GUS was used as the experimental material.The empty vector homozygous Arabidopsis thaliana(VT)and homozygous wild-type Arabidopsis lines(WT)were used as controls,targeted and selective to some of the above strains of Arabidopsis thaliana under abiotic stress.Morphological,physiological,and molecular indicators were examined and the following experimental results were obtained.The results of the morphological indicators such as seed germination rate,seedling root length,plant height,number of pods,and survival rate showed that transgenic Sl NAC6 Arabidopsis had stronger seed germination ability,root elongation ability,reproductive ability,and survival.ability.The chlorophyll fluorescence,relative conductivity,malondialdehyde content,proline content,SOD activity,POD activity,and CAT activity of these physiological indicators showed that transgenic SlNAC6 Arabidopsis plants had stronger photosynthetic efficiency and were able to Better protection of the integrity of the cell membrane structure,better osmoregulatory capacity and higher scavenging capacity of reactive oxygen species,thereby reducing the degree of destruction of cell morphology and function of the transgenic Arabidopsis lineage,ensuring intracellular physiology Biochemical metabolism proceeds normally.Twelve Arabidopsis genes RD29 A,RD29B,RD20,SGR2,ERD10,COR47,PORA,IAA6,MYB96,VSP1,AZF1 and GSTF6 related to growth,development and stress were selected by real-time quantitative PCR(qRT-PCR).The relative expression of each gene in transgenic Arabidopsis line L1 and wild type Arabidopsis line WT under normal culture conditions was examined.The results showed that compared with wild-type Arabidopsis thaliana,theexpression levels of 11 genes transfected with SlNAC6 gene Arabidopsis thaliana were up-regulated to varying degrees,and only MYB96 gene expression was down-regulated.Thus,Sl NAC6 may improve the resistance of transgenic Arabidopsis through regulating the expression of other stress-related functional genes.This study shows that SlNAC6 regulates the expression of other transcription factors and related functional genes through their own overexpression,which protects or strengthens the physiological metabolism of the plants against the stress of the environment,resulting in the transgenic plants exhibiting a more excellent phenotype,and finally transgenes.Plants show greater resistance to adversity stress.This study provided new ideas for the study of plant stress resistance mechanism.