Analysis On Stress Resistance Of SlNAC4 Transgenic Arabidopsis And Its Mechanism

Abiotic stresses such as high salt,drought,low temperature and other environments will affect plants growth and development,on the contrary,plants will also form a variety of regulatory mechanisms to adapt and resist abiotic stress,in these mechanisms,The NAC transcription factors play a very important role.In this paper,the morphological,physiological and molecular indexes of abiotic stress were tested to reveal the ability and mechanism of resistance to stress in the SlNAC4-transgenic Arabidopsis.The results as follows:In the morphological structure,we detected the germination rate,root length,lateral root number,survival rate,growth state and stomatal opening rate of different genotypes Arabidopsis thaliana.It was found that the SlNAC4-transgenic Arabidopsis with stronger seed germination ability,root water absorption capacity,plant viability and growth ability.It was proved that the translocation of SlNAC4 gene had better growth status and viability in Arabidopsis thaliana under abiotic stress.In the physiological structure,we detected the electrical conductivity,MDA,SOD,POD,CAT,proline and betaine of different genotypes Arabidopsis thaliana,and found that the Sl NAC4-transgenic Arabidopsis had a more complete membrane structure,Oxidation capacity and osmotic adjustment capacity.At the molecular level,we screened and detected a total of 3094 differentially expressed genes by gene chip.Six genes were selected for real-time quantitative PCR and found to be consistent with the expression of gene chip,which proved that the gene chip was reliable.In these differential genes,AT2G38240 is related to seed germination and organ maturation.RNS2 is related to intracellular osmotic pressure and membrane structure stability.DFR,GSTF6 and NRT2.6 are related to antioxidant enzyme activity.PIP5K3 is associated with root Length and lateral roots;ORA59 is related to root length and osmotic adjustment material;EXPA8 is related to porosity switch.Several representative transcription factor families,MYB and WRKY,were also found.SlNAC4 may improve resistance by regulating the expression of MYB and WRKY transcription factors.This study revealed the role of SlNAC4 in improving the plant stress resistance,and analyzed the downstream genes regulated by SlNAC4.It was thought that SlNAC4 wasregulated by AT2G38240,DFR,GSTF6,NRT2.6,EXPA8,MYB and WRKY genes or transcription factors,Enhanced the growth and development ability of transgenic Arabidopsis thaliana and the ability to resist abiotic stress,and provided new ideas for plant resistance mechanism research.