Study On Genetic Polymorphism Of 20 STR Loci In Han Population In Mudanjiang Area

Objective:To obtain genetic data of Han people which included genetic frequencies in Mudanjiang region,Heilongjiang Province,the genetic polymorphisms of short tandem repeats(STR)loci in DNA of 1,399 healthy Han Chinese were studied by PowerPlex 21 System fluorescence amplification kit,and data analysis was performed.These results ccould provide basic data for the STR gene database of Han population in the north of China.At the same time,it also provided reliable reference data for the genetic frequency difference among the Han population in China,thus laying a theoretical foundation for individual identification and paternity identification of the population.Methods:In the experiment,1399 human blood cards of Han residents in Mudanjiang area of Heilongjiang which had no direct relative relationship were selected.After DNA extraction,PCR amplification,capillary electrophoresis with 3100 X L genetic analyzer,and GeneMapper ID V 3.2 analysis,the STR typing was obtained.Hardy-Weinberg equilibrium(HWE)test were carried out by Modified-PowerStats software.The heterozygosity(H),polymorphic information content(PIC),individual identification rate(DP),unrelated individual identification rate(Pm),non-paternal exclusion rate(PE),typical paternity index(TPI),cumulative individual identification rate(TDP),and cumulative non-paternal exclusion rate(CPE)of each loci was obtained by calculation.Results:By analyzing the autosomal STR data which were obtained from 1399 samples,all loci except the sex locus was examined.On the 20 autosomal STR loci which including D3S1358,D1S1656,D6S1043,D13S317,Penta E,D16S539,D18S51,D2S1338,CSF1 PO,Penta D,TH01,vWA,D2S11,D7S820,D5S818,TPO11,TPO11,T819,D817,D12391 and FGA,10,17,19,9,26,8,20,13,11,12,11,9,21,11,10,9,11,14,16,and 23 alleles were detected,respectively.Meanwhile,25,73,88,30,165,26,86,69,29,49,26,31,81,37,38,22,44,66,62,and 96 genotypes were also examined in the corresponding locus,respectively.After analyzing,20 STR loci were all consistent with HWE.The H value of each loci was between 62.7%and 91.0%,the DP value was from 0.799 to 0.987,the PIC value was between 0.57 and 0.91 which were more than 0.5,the PE value was from 0.324 to 0.816,and the TPI value was between 1.34 and 5.55.The cumulative TDP was 1-7.86 x 10-25 and CPE was 0.9999996 which were calculated by using the gene frequency that were obtained from this experiment.Conclusion:Among the 20 gene loci tested,18 loci including D3S1358,D1S1656,D6S1043,D13S317,Penta E,D16S539,D18S51,D2S1338,CSF1,Penta D,vWA,D21S11,D720,D5S818 and DDDS79 had higher heterozygous degree and discrimination force than TH01 and TPOX.Therefore,the PowerPlex(?)21 System Fluorescence Amplification kit used in this test was very effective in forensic paternity and individual identification in Mudanjiang region of Heilongjiang Province.The data of genetic polymorphism of 20 STR loci in the Han population of Mudanjiang,Heilongjiang province,was not exactly the same as those obtained from other areas,whether from allele frequency,allele genotyping or discriminative power of each loci.To a certain extent,this results reflected that the genetic polymorphism data of Han population in Mudanjiang did not conform to the genetic characteristics of Han Chinese in some respects.Further research could be undertaken in areas such as human genetics.At the same time,the data obtained from this experiment also provided reliable basic data for the study of STR genetic polymorphism in Han population,and provided new basic data for the construction of forensic science DNA database and population genetic database,and also provided new genetic parameters for the identification of forensic individuals and the parental authority in Mudanjiang region of Heilongjiang province.