Genetic Polymorphism Of Four STR Loci D4S3489, D5S2970, D13S1891,D15S1549 In Chinese Population

Objective: We focus on exploring novel STR loci : D4s3489,D5s2970,D13s1891 and D15s1549 in order to develop a set of new markers for forensic genetics and tostudy the four STR loci for genetic application.Material and Methods: The EDTA-blood samples were collected from 300 unrelatedindividuals in Chinese Population. Genomic DNA was extracted from fresh blood byuse of standard Phenol-chloroform Protocols. Each DNA sample was amplified usingPCR for D4S3489, D5S2970, D13S1891, D15S1549 loci. DNA was separated onnative horizontal discontinuous 8.0% polyacrylamide gels as described and bandswere visualized by silver. Statistical processing of the results was analyzed usingPOWERSTATS program. The exact test of the Hardy-Weinberg equilibrium(1 000 000 steps in Markov chain) was performed using arlequin software version3.11. With these programs, the heterozygosis, power of discrimination (PD) parentage,polymorphism information content, probability of exclusion for complete andmatching Probability were calculated.Results: 1.D4s3489,D5s2970,D13s1891 and D15s1549 loci were accordance withthe Hardy-Weinberg -equilibrium(p>0.05) in Chinese population.2. Heterozygosis of these loci is all higher than 70% except for D15S1549:0.6860.And PIC are all higher than 0.7 besides D5s2970.3.The discriminating power of the four were0.959,0.965,0.842,0.934, The exclusionprobability was calculated as 0.527 for D4S3489and 0.581 for D13S1891 and 0.465for D5S2970 and 0.406 for D15S1549.Conclusions: The distributions of allelic frequencies at four loci are satisfying in population studied. Present data suggest that D4s3489,D5s2970,D13s1891 and D15s1549 loci can be applied as new candidate markers for forensic individual identification and paternity testing.