The Effect Of Traditional Chinese Medicine Application Of "Winter Disease And Summer Treatment" On The Immune Function Of Dendritic Cells In Asthmatic Rats

BackgroundAsthma is the most common chronic respiratory disease in children, is a variety of inflammatory cells and medium interaction of chronic inflammatory airway diseases, recent studies showed asthma Thl/Th2 cell is the most important immune abnormalities and functional imbalances. DC is known as the most potent antigen-presenting cells, in addition to a unique ability to stimulate the proliferation of t cells, but also directly affect the differentiation of ThO cells, leading to Thl/Th2 imbalance caused by asthma. "Winter disease in summer treatment" therapy of acupoint drug delivery are the most typical representative of the prescription, through the channels and collaterals acupuncture point stimulation and the dual role of drug absorbed, achieve the purpose of treatment of asthma. Study on the induction of DC method in combination with the past culture on the basis of, the "winter disease in summer treat" acupoint therapy can cut the expression of dendritic cells, inhibition of allergic inflammation of asthma, envisaged to reduce asthma attacks, asthma treated by acupoint. application therapy may explore the immune mechanisms associated with DC.Objective:Verify that "winter disease in summer treat" acupoint therapy can by reduced expression of dendritic cells, inhibiting the allergic inflammation of asthma, enhance the immune function of dendritic cells, thereby reducing the idea of asthma attacks.Method:Egg white protein (VOA) and asthma in sensitized rats model with ELISA method for detection of serum OVA IgE levels, acupoint Group asthma model group, there are no differences between control group; with trachea was examined by the bronchoalveolar lavage fluid (BALF) levels of IL-4, IL-5, there is no difference between the groups. Through the DC stable cultivation method of lungs in rats by flow cytometry lung DC surface molecules expression of CD11cCD80,CD11cCD86, dendritic cell surface molecules expression level in each group there is no difference.Result:Compared with the control group, mode1 dendritic cell surface molecules expression level (p<0.05), compared with the model groups, acupoint group decreased dendritic cell surface molecules expression level (p<0.05).Conclusion:Acupoint therapy can by reduced expression of dendritic cells, suppress asthma allergic inflammation, to reduce asthma attacks.