Preparation Of Yimucao Granules And Evaluation Of Its Clinical Efficacy

Motherwort is labiatae, Leonurus artemisia genus plant, with promoting blood circulation and regulating the menstrual, reducing fever and causing diuresis and detumescence by detoxification effect. Modern pharmacological research show that Motherwort can improve the body of ovarian haemal circulation, regulate the level of sex hormone and promote the secretion of sex hormones. Stachydrine hydrochloride is one of the main effective components of alkaloid, high content in leonurus. Motherwort herb is Veterinary Veterinary Drug in gynecology of conventional varieties, the main method used is administered in the powder directly mixed with feed or boiling water. With the rapid development of intensification and large-scale breeding of domestic animals, spices or boiling administration ways has been unable to use, Motherwort urgent need to change the current dosage, and the establishment of a modern quality standards. This study explored the preparation process of Motherwort granules, establish quality standards for its granules, and evaluate its clinical efficacy, Motherwort lay the foundation for the development of new veterinary drugs.First tests with hydrochloric acid Stachydrine evaluation index, single factor test and orthogonal test optimum extraction of motherwort, through materials selection and particles Rating granules optimization of the molding process. Secondly, To establish the determination standard Motherwort particles with HPLC-ELSD, Establish qualification standards of Motherwort granules with thin layer chromatography. Finally, with the clinical trials to evaluate the efficacy of Motherwort particles for farrowing female mice and sows. Test results are as follows.1 ? Motherwort Optimization of Extraction Process: using the technology of decoction method, Optimum conditions, water ratio is 20: 1?Extracted twice?1.5 hours extract time.2 ? The optimization of Motherwort particles forming process: Concentrated motherwort concrete to a relative proportion of 1.1 to 1.2, Wet granulation, orthogonal test showed the optimum ratio of materials and varieties, extract: starch: dextrin = 1: 1: 2.3 ? Motherwort particles quality standards: TCL is based on the best conditionsacetone-ethanol-HCl(10:6:1) as the agent, 105 ? expand 20 min, sprayed with chromogenic reagent dilute bismuth potassium iodide test solution-ferric chloride solution(10:1), showing clearly the position of the target spot. Motherwort particles using HPLC-ELSD Venusil HILIC hydrophilic column(250 mm × 4.6 mm, 5 ?m), The mobile phase was acetonitrile-0.2% acetic acid(70:30), flow rate of 1.0 mL/min,column temperature was 30 ?, Detector drift tube temperature 80 ?, the atomization chamber temperature 50 ?, carrier gas volume flow 1.0000 mL/min. Regression equation for Srachy-drine Hydrochloride was Y=1.6575x+5.1338, R2=0.9993,Srtachydrine Hydrochloride deteched was stable during 0.50~5.00 ?g/mL.4 ? Evaluation of clinical efficacy standards of motherwort particles : The test results show, Motherwort particles can Organ index postpartum females in the experimental group was significantly higher(P <0.05), Tissue sections showed an increase in immune organs, germinal center becomes large, good recovery of the reproductive system and reduce inflammation. and also can shorten, the time of postpartum discharge lochia sow about 0.56 days, Early weaning estrus approximately2.3 days and 4.5% increase the survival rate of piglets weaned.In summary, Motherwort extract reasonable conditions, viable process for preparing granules, quality standards controllable, stable product quality, With the advantages of using, storage, easy to carry. add Motherwort granules in the diet, can improve postpartum sow performance and disease resistance, improve maternal immunity performance, accelerate the rehabilitation of their reproductive system.