Clinical Efficacy And Safety Of Hydrochloride Valnemulin Premic In Swine

The author studies the efficacy and safety tests of hydrochloride valnemulin premix clinical, its content mainly includes:the in vitro antiacterial experiment of hydrochloride valnemulin premix, acute toxicity tests, target animal safety tests and clinical curative effect validation, provide test data and the relevant scientific basis for its new veterinary drug approval.First, do the microbial sensitivity test with the certain concentration of drugs and bacterium fluid filtration according to trace dilution method. The results show that, the bacteriostatic activity of hydrochloride valnemulin paires to staphylococcus aureus and salmonella choleraesuis is strong, MIC respectively is:0.031 and 0.063μg/mL, but the bacteriostatic activity to escherichia coli, pap bacillus and Streptococcus suis is weak, the MIC respectively is 4,2,16μg/mL, its bacteriostatic activity to Pleural pneumonia defence coli and mycoplasma(MIC respectively is 0.031,0.0025μg/mL) is far better than Tiamulin Hydrogen Fumarate(MIC respectively is 0.5,0.05μg/mL) and enrofloxacin base (MIC respectively is 1,0.5μg/mL). Moreover the bacteriostatic activity of hydrochloride valnemulin paires to escherichia coli and pap bacillus is weaker than the one of Tiamulin Hydrogen Fumarate. But the bacteriostatic activity to staphylococcus aureus, Streptococcus suis, salmonella choleraesuis and pap bacillus is better than the one of Tiamulin Hydrogen Fumarate.And the bacteriostatic activity to escherichia coli is near to Tiamulin Hydrogen Fumarate.Secondly, design the experiment in acute toxicity tests of hydrochloride valnemulin with Karber's method to get the test team.there are six examined groups in experiment, the grouping interval is 0.15, besides infer LD100=3235mg/kg, LDo=576mg/kg of hydrochloride valnemulin. According to the datas take official test. Then observe the toxic reaction of experimental mice and rats after using hydrochloride valnemulin with oral. Finally, we can get the LD50 of rats and mice (respectively is 1374.4mg/kg and 1611.6mg/kg) after intragastric administration with hydrochloride valnemulin.The result shows that hydrochloride valnemulin is low-toxic according to the toxicology classification.There are four groups in animal safety tests. Including dose groups containing hydrochloride valnemulin 200g/tfeed,600g/t and 1000g/t and a blank check experiment group. After three weeks feeding, observe the toxic reaction of clinical pigs after the excessive use of hydrochloride valnemulin. Compare results to blank check experiment group, results show that pigs in the group of 1000g/t dose, the feed intake depress, but do not reach significant change. Hydrochloride valnemulin also does not significantly affect to pig of the daily gain and the rate of feed and weight (p> 0.05). Besides hematology and blood biochemical index also are not significantly influenced. In two high dose groups (600g g/t and 1000g/t), feed intakes of pig is slightly cut down. Furthermore ALT and AST have increased, but ALP has a lower trend.It shows that using hydrochloride valnemulin with high dose have certain effect to pigs blood biochemical indicators.Finally, the author validated efficacy of 10% hydrochloride valnemulin premix to Mycoplasma Pneumonia, porcine contagious pleuropneumonia and pig dysentery in clinical efficacy trials.According to the test results, propose that clinical therapeutic dose for treatment of Mycoplasma Pneumonia and porcine contagious pleuropneumonia is 200g 10% hydrochloride valnemulin premix in 1000kg feed, feed the experimental animals for 14-21 days; clinical therapeutic dose for treatment of pig dysentery is 75g 10% hydrochloride valnemulin premix in 1000kg feed, feed the experimental animals for 14-21 days.