The Fabrication Of CuCNFs Composite Nanofibers And Their Study In Hydroquinone Biosensor

The hydroquinone exists in waste water has aroused great attention due to their toxicity which can not be neglected. It is significant to monitor the hydroqinone in waste water. Biosensor was applied to the monitoring for its high sensitivity, ideally portable which is beneficial for online monitoring, and fast response. The search for new advanced materials is an important area of contemporary research in the development of biosensor. Nano-structured materials of different chemical composition have attracted great attention, including their formation, properties and application. In this work, we prepared three novel composite nano materials based on CuCNFs composite nanofibers and applied them to laccase based biosensor for the detection of hydroquinone in waste water. Details are as follows.A novel biosensor was prepared on the basis of laccase, Nafion and TiO₂ loaded copper and carbon composite nanofibers?TiO₂/CuCNFs? obtained through electrospinning, carbonization and solvothermal treatment. The introduction of TiO₂ apparently improved the electrocatalysis of the biosensor for the detection of hydroquinone. In addition, cyclic voltammetry analysis of the biosensor showed a pair of well-defined redox peaks, and revealed that the electrochemical behavior of laccase was a surface-controlled process. Chronoamperometry technique was also employed to investigate the biosensor. The results displayed a linear range from 1 ?M to 89.8 ?M, detection limit of 3.65 ?M?S/N = 3?, a sensitivity of 24.6 ?A/mM, a high selectivity, as well as good repeatability and stability.A novel nanomaterial composed of CuCNFs composite nanofibers decorated with Ag doped TiO₂?Ag-TiO₂? nanoparticles was prepared through electrospinning, carbonization and solvothermal treatment. The composites were characterized by scanning electron microscopy?SEM?, transmission electron microscopy?TEM?, X-ray diffraction?XRD?, Fourier transform infrared spectroscopy?FTIR? and electrochemical impedance spectroscopy?EIS?. The obtained composites were mixed with laccase and Nafion to construct novel hydroquinone biosensor. The electrochemical behavior of the novel biosensor was studied using cyclic voltammetry?CV? and chronoamperometry. The results demonstrated that the biosensor possessed a wide detection linear range?1.20 – 176.50 ?M?, a good selectivity, repeatability, reproducibility and storage stability.We presented a facile and efficient method to synthesize PANI/CuCNFs and applied them to the laccase based biosensor for the detection of hydroquinone. The morphology and composition of the fabricated nanocomposite were characterized by scanning electron microscopy?SEM?, transmission electron microscopy?TEM?, energy dispersive X-ray spectroscopy?EDX?, X-ray diffraction?XRD? and Fourier transform infrared spectroscopy?FTIR?. The electrochemical behaviour of the novel biosensor was studied using electrochemical impedance spectroscopy?EIS? and cyclic voltammetry?CV?. The results showed that the constructed biosensor possessed high selectivity, reproducibility, repeatability, sensitivity?41.65 ?A/m M? and a low detection limit?2.37 ?M?. The linear range of the biosensor was from 500 nM to 110 ?M.