| The study of bionic membrane involved various disciplines, including molecular biology, biochemistry and materials chemistry, is one of the fastest growing frontier fields. The preparation of "biological membrane" by simulating structure and function of biomembrane at the molecular level will derive new concepts and new technologies about chemical materials and bio-engineering materials. In a word, the study of bionic membrane is constructing biomimetic membranes materials with similar structure and function, based on strong sense of the structure and function of biofilm composition materials, particularly phosphate, liposomes, membrane proteins and their combination. With the development of the study, more and more synthesis mechanisms of bionic membrane are being revealed in various aspects and the synthesis technology of bionic membrane will be more mature. The prospect of bionic membrane appears unlimited.The preparation, selection and synthesize of of bionic membrane were introduced, and the progress of protein modified electrode was also reviewed in detail in this article. The main research works are shown as follows:1. Mesoporous SBA-15 was synthesized by using a hydrothermal method. Cytochrome c was immobilized on SBA-15 modified glassy carbon electrode. The interaction between Cytochrome c and SBA-15 was examined by using UV-Vis spectroscopy and electrochemical methods. The direct electron transfer of the immobilized Cytochrome c exhibited a pair of redox peaks with formal potential (E0’) of about 0.275 V (versus SCE) in 0.1mol/L pH 7.0 PBS. The electrode reaction showed a surface-controlled process with the apparent heterogeneous electron transfer rate constant (ks) of 2.25 s-1. Cytochrome c immobilized on the SBA-15 modified electrode exhibits a remarkable electrocatalytic activity for the reduction of hydrogen peroxide (H2O2). Based on these, a third generation biosensor can be constructed for the determination of H2O2.2. The direct electrochemistry of Cytochrome C(Cyt C) immobilized on nanoparticle Mn3O4 modified glassy carbon electrode was investigated by cyclic voltammetry (CV). The results show that a pair of well-defined quasi-reversible redox peaks of Cyt C was obtained at Nafion/Cyt c/Mn3O4/GCE based enzyme electrode by the direct electron transfer between the protein and the electrode. The peak current was linearly dependent on the scan rate, indicating that the direct electrochemistry of Cyt C in that case was a surface-controlled process. Mn3O4 fixed in the Cyt c can promote hydrogen peroxide catalytic reduction. The experimental results can provide a basis for a new type of sensor.3. A new type of H2O2 biosensor based on the direct electron transfer was constructed by modifying Cyt c on the Nafion and Fe2O3 particles. The direct electron transfer of the immobilized Cyt c exhibited a pair of quasi-reversible redox peaks with formal potential (E0’) of about 0.220 V (vs SCE) in 0.1mol/L pH 7.0 PBS. The electrode reaction showed a surface-controlled process with the apparent heterogeneous electron transfer rate constant (ks) of 1.21 s-1. The modified electrode showed excellent electro-catalytic activity toward H2O2 in the range of 2.0×10-6 mol/L-3.0×10-3mol/L, and its detection limit, apparent Michaelis-Menten constant are 1.0×10-6 mol/L and 1.35 mmol/L. The modified electrode exhibited good affinity.4. Attapulgite(ATT) was modified with cetyltrimethylammonium bromide(CTAB), and adhered to a glassy carbon electrode by physical absprption to fabricate CTAB/ATT modified glassy carbon electrode. The electrochemical behaviors of the three kinds of hydroquinone on the modified electrode were investigated. The results show that the electrode has good sensitivity,selectivity and stability for hydroquinone. Under the optimum experimental conditions, the oxidation peak current has a linear relationship with concentration of hydroquinone. We established a new method of differential pulse voltammetry for determination of three kinds of hydroquinone. |
PDF Full Text Request