Self-Reference Surface Plasmon Resonance (SPR) Fiber Biosensor

Surface plasmon resonance (SPR) has become a new technology for label-free detection and biochemical detection. Due to the miniaturization, portable, cost-effective, remote and real-time detection high sensitivity and fast response, SPR has been extensively investigated for chemical and biological sensing applications.On account of the high sensitivity and stability of the fiber-optic SPR sensor, it has a high requirement for the external environment while remote biological and environmental detection in an external environment. However, the environmental condition (bulk refractive index, external temperature) will influence the performance of SPR sensor for in situ measurement, thereby reducing the stability and accuracy of the sensor. So self-reference SPR sensor gain widespread attention in recent years.This thesis is focus on self-reference SPR sensing technology, which includes the following major works:Firstly, this paper describes the features, application and history of SPR technology development, and states the present research situation and future development of the SPR sensor. Then, we theoretically introduce the sensing theory of SPR and design the software development platform of virtual instrument based on the software LabVIEW. Aiming at the several different SPR biosensors we designed in this paper, we design and build a SPR sensing system. In addition, the primary element and their producing technology are introduced in this paper.Next, several self-reference SPR biosensors with different operating principle and sensing element construction are presented and then the research model of our self-reference fiber-optic SPR sensor is proposed, which can realize bulk refractive index or external temperature compensation. The compensation characteristic and the biochemical detection capability of these SPR biosensors can be demonstrated by experiments.Finally, combining with the above we design a self-reference SPR biosensor, which can compensate the influence of bulk refractive index and external temperature simultaneously. We experimental test the compensation and biochemical detection function of the fiber-optic SPR sensor. Its fabrication process and performance is also presented. The experiment result shows the referencing signal can be used to compensate for SPR measuring signal caused by system instability.In conclusion, in comparison to the self-reference SPR sensor reported previously, the self-reference fiber-optic SPR biosensors we proposed has many advantages for biochemical sensing, such as low cost, simple fabrication, reproduction and no receptor needed. This kind of fiber-optic SPR biosensor can become a good candidate in the field of self-reference SPR sensing measurements.