Study On The Establishment And Evaluation Of A New Type Rabbit Abdominal Wall Hernia Model

Objective: To establish a more rapid and stable new type of rabbit abdominal wall hernia model,and explore its evaluation criteria through animal experiments,and to verify the possibility of expansion and repair of hernia repair in animal models.Methods: 1.50 adult male rabbits were randomly divided into five groups,A,B,C,D and E,with 10 in each group.The A group was a blank control group,and the other four groups were in the experimental group.The four groups in the experimental group were abdominal median resection of the diameter of 3cm round full-thickness abdominal wall tissue,but each group was different in the way of resection and modeling.In group C,the hernia ring was artificially created in operation,and E group was artificially constructed hernia sac and hernia ring during operation.After the experiment,we observed the hernia maturation from 28 days to the abdominal wall,and observed the herniation of the abdominal wall(the time and size of the lump block)and the whole body condition of the rabbit(including body weight,nutrition,wound infection and death).After 28 days,the abdominal cavity adhesion of the experimental group was opened.2.After the best modeling method was obtained,hernia repair was performed on the experimental group,and the hernia sac,hernia ring and the space of muscle tissue were observed.Results: 1.There was no significant difference in body weight between the experimental groups at each time point(P>0.05).The weight change trend was the same within 28 days after operation.2.The abdomen of the rabbits after the operation showed a prominentmass,and was significantly earlier in the D and E groups than in the B and C groups.There was no significant difference between the B and C groups(P>0.05).Between group B and D,the difference between groups B and E,between groups C and D,and between groups C and E was statistically significant(P<0.05).The postoperative complications and mortality of group D were significantly higher than those of other experimental groups.3.After 28 days,the intraperitoneal adhesions of the experimental groups were observed.C and E groups were milder than those of the B and D groups.According to the Qiao’s adhesion grading method,there was no significant(P>0.05)difference between the groups B and D and between the C and E groups.between group B and C,between group B and E,between group C and D,and between group D and group E were statistically significant(P<0.05).4.In the E group,the pre-myelin repair operation(Onlay method)was successful.During the operation,the hernia sac and hernia ring were obvious and typical,and the muscle clearance could be clearly separated.Conclusions: 1.This study proposes a method for establishing a rabbit abdominal wall hernia model more quickly and steadily.It can provide an animal model for future research on new abdominal wall hernia and new repair materials.2.The abdominal wall hernia model was repaired by the common clinical procedures and the clear anatomical structure was seen during the operation,which provided feasibility for the development of new surgical procedures and new repair materials in the future.