Yi Gas-solid Table Pill For COPD Acute Heavy-duty Frequently (lung Spleen Deficiency Syndrome)on Patients With Sputum Supernatant Of AQP1,the Influence Of AQP5 Expression

Objective: Observation of Yiqi Gubiao pill for COPD frequent acute exacerbations(lung and spleen deficiency syndrome)regulating AQP1 and AQP5 expression in patients with sputum supernatant,trying to explain the mechanism of the effect of Yiqi Gubiao pill in the treatment of COPD.Methods: into the 2011 accord with GOLD revision diagnostic criteria and diagnostic criteria of stability often acute heavy-duty(lung spleen deficiency syndrome)patients in 112 cases;The healthy check-up 20 cases as healthy controls.Use prospective,randomized,double-blind,controlled clinical trial method will collect objects for COPD acute heavy-duty frequently(lung spleen deficiency syndrome)patients(112 cases),according to the proportion of 1:1 were randomly divided into two groups,conventional western medicine therapy or western medicine routine add profit gas-solid table group,12 weeks after treatment,the participants of induced sputum samples.Observation of sputum smear(HE)staining,count of neutrophils,lymphocytes,and the percentage of the number of eosinophils cells,method of enzyme-linked immunosorbent(ELISA)determination of AQP1,AQP5 in sputum supernatant on content.Results: compared with the healthy group,frequent acute heavy-duty gas-solid table groups,frequent in patients with acute heavy-duty western medicine treatment group sputum samples of neutrophils,lymphocytes,eosinophils number and percentage were significantly higher than that of healthy people(P < 0.05),sputum that the expression of AQP1 in the supernatant fluid analysis: as the patients with COPD of acute heavy-duty frequently,western medicine treatme nt group and add beneficial gas-solid table pill group compared to the former is less than the latter,comparing the two groups have significant difference(P < 0.01);Western medicine treatment group compared to healthy controls a significant reduction in the concentration of AQP1 expression,with significant difference(P < 0.01);Take a profit gas-solid table pill group compared to healthy controls,the former AQP1 concentration is higher than the latter,comparing with significant difference(P < 0.01).Sputum that AQP5 content in the supernatant fluid of expression: western medicine treatment group compared with add profit gas-solid table pill group expression of AQP5 concentration significantly,with significant difference(P < 0.01);Western medicine treatment group compared with no obvious difference in the concentration of the health control group AQP5 express no statistical significance(P > 0.05);Take a profit gas-solid table pill group compared to healthy controls,the expression of the former AQP5 concentration higher than the latter,comparing with significant difference(P < 0.05).Conclusion: As an effective preparation for the treatment of COPD,supplementing qi and consolidating table pill is not clear about the time interval and the mechanism of the prolongation of the COPD stable phase.Through the study found that the AQP1 and AQP5 expression could increase in COPD patients with sputum supernatant,suggesting that Yiqi Gubiao pill its mechanism may regulate the effect of AQP on airway inflammation,relieve the airway and improve ventilation function.