Dendritic Cells In The Induced Sputum Of Patients With Chronic Obstructive Pulmonary Disease

Objective In the present study,the function of dendritic cells was investigated in the pathogensis of chronic obstructive pulmonary disease(COPD) through the change of dendritic cells in the induced sputum of patients with COPD.Methods The cells count determined by CD40 and CD86 were analyzed with flow cytometry in the sputum collected from COPD Groups,Cigarette Smoke Groups and Control Groups.FEV1%and FEV1/FVC were determined,and the correlation between DCs and FEV1%was analyzed in all subjects.Results Flow cytometric analysis of DCs in the GOLDⅠ～Ⅳof COPD groups showed the upregulation of both CD40 and CD86,compared with DCs in Cigarette Smoke Groups and Control Groups.There was negative correlation between CD 40 and FEV1%(r=-0.913,P＜0.05).There was also negative correlation between CD86 and FEV1%(r=-0.893,P＜0.05).Conclusion.The significantly upregulation of CD40 and CD86 in the patients with COPD and the positive correlation between CD40,CD86 and the stage of COPD showed that DCs maybe contribute to the inflammatory reaction of COPD.