| BackgroundThis article of Junan County women's serum in serum calcium, copper, magnesium, iron, zinc, boron, chromium, manganese, cobalt, nickel, arsenic, selenium, cadmium, lead etc 14 kinds of elements content and common gynecological disease do related analysis. Hope to provide effective help on medical intervention and improve the happy index of women.Objectives1?To investigate the level and distribution characteristics of serum elements (calcium, copper, magnesium, iron, zinc, boron, chromium, manganese, cobalt, nickel, cadmium, lead, arsenic, selenium, etc) of female aged 15 to 49 years old in Junan county.2?To investigate the health status of female aged 15 to 49 years old in Junan county.3.?To analyse the relevance between the level of 14 kinds(calcium, copper, magnesium, iron, zinc, boron, chromium, manganese, cobalt, nickel, cadmium, lead, arsenic, selenium) elements with women's health.Methods1?InvestigationAll permanent female residents aged 15 to 49 years old of six villages drawn from Junan with cluster sampling were investigated. Household survey was selected to investigate.9mL of anticoagulant blood was centrifuged with 1000 rpm for 5 to 10 minutes, and the serum was placed into eppeddorf tubes. Then the tubes were numbered, transported and kept in low temperature.2?Determination of serum samplesThe methods to determined serum levels of elements by atomic absorption spectrometry in defined instrument conditions and sample processing were tested. The detection limits, precisions including inter-day precision and intra-day precision, relative standard deviation and add mark recovery rates were calculated and assessed. After processed, the serum samples were successively determined for five kinds of elements(calcium, copper, magnesium, iron, zinc)content by atomic absorption spectrometry. The serum concentration of elements could be calculated by multiplying the dilution factors (ten times).The methods to determined serum levels of elements by inductively coupled plasma mass spectrometry in defined instrument conditions and sample processing were tested. The detection limits, precisions including inter-day precision and intra-day precision, relative standard deviation and add mark recovery rates were calculated and assessed. After processed, the serum samples were determined for levels of elements by inductively coupled plasma mass spectrometry. The serum concentration of elements could be calculated by multiplying the dilution factors. After dilution with 0.1%Trition-X-100+0.5%NH4OH by a factor of 10, the serum was directly determined by ICP-MS which use external standard method to calibrate the instrument work cure. After processed, the serum samples were successively determined for nine kinds of elements (boron, chromium, manganese, cobalt, nickel, cadmium, lead, arsenic,selenium) content by collision cell inductively coupled plasma spectrometry. The serum concentration of elements could be calculated by multiplying the dilution factors (ten times).3?Quality controlQuality control was required to be done from investigation and sampling to determination of laboratory indicators and data input. Selection principle of respondents was confirmed before the survey. Then quality control was also done along with the investigation and samples collecting. The laboratory indicators were determined within the acceptable range of laboratory variation. All reaction reagents were carefully to control from pollution and the ultrapure water was used in the whole experiment process. Then quality control was also done along with samples collecting. The laboratory indicators were determined within the acceptable range of laboratory variation by making the quality control chart. A new standard curve was fitted per 20 samples as determination of serum trace elements.4?Data processingA database was established and the data from experiment and survey was entried. And then the required data was statistically processed.Statistical software of SPSS 20.0 was used and the data was presented as mean± standard deviation(x ± s).The used statistical approaches included independent samples t test, chi-square test, analysis of variance, simple correlation. It was considered statistically significant if P<0.05.Results1?To verify the flame atomic absorption spectrophotometric method determination of serum calcium, copper, magnesium, iron, zinc content of five elements, the results show that this method can meet the requirements of determination. The detection limit of elements Cu, Fe, Zn, Ca and Mg were 0.02 mg/L, 0.03 mg/L,0.01 mg/L,0.05 mg/L,0.004 mg/L; The intra day precision were 0.97%, 1.06%,1.89%,2.09%,1.84%, the inter day precision were 3.22%,3.79%,3.95%, 4.17%,4.25%, Intra day and inter day precision were less than 10%; the relative standard deviation were 1.61%,1.41%,1.77%,1.50%,1.19%; Recovers of each element low, medium, high were between 85%-110%.2?To verify the collision pool inductively coupled plasma mass spectrometry (ICP-MS) method for the determination of serum boron, chromium, manganese, cobalt, nickel, arsenic, selenium, cadmium, lead 9 kinds of element content to validate the methodology. The results show that this method can meet the requirements of the elements in the analysis of serum. The detection limit of B, Mn, Co, As, Ni, Se, Cd, Pb and Cr were 0.200 g/L,0.005 g/L,0.038 g/L,0.004 g/L,0.037 g/L,0.363 g/L, 0.392 g/L,0.042 g/L,0.002 g/L, recovers of each element low, medium, high were between 85%-115%, low, medium and high intra day precision each element were less than 5%, low, medium and high inter day precision each element were less than 15%, The relative standard deviation were 1.20%,1.62%,1.33%,1.72%,1.56%,1.49%, 1.82%,1.52%,1.94%, the correlation coefficient R of each elements is better than 0.99.3?T Test the element content in serum and with menstruation or not of 725 research target, there was no statistically significant difference.(P>0.05). No significant correlation between serum elements and menarche age. Do t test about element content and menstruation regular or not. Boron in serum of regular menstruation group(54.20±25.78?g/L) is significantly higher than not regular group (46.32±22.19?g/L) (P<0.05). Serum elements and days of the menstrual cycle and menstrual days were not significantly related. The Cr and Se content of often dysmenorrheal is 1.45±0.51?g/L and 108.12±28.99?g/L, the Cr and Se content of occasionally dysmenorrheal is 1.72±0.57?g/L and 119.15±29.24?g/L, the Cr and Se content of no dysmenorrheal is 1.76±0.52?g/L and 121.15±30.74?g/L. The difference was statistically significant (P<0.05). Data show the arsenic content in serum of stop group (2.05±1.81?g/L) and no stop group((1.56±1.03?g/L)) have significant differences. The survey respectively according to whether the pill and whether patients with pelvic inflammatory disease or vaginitis of independent sample t test, there was no significant difference. The Cr (r=0.11, P<0.05) and Ni (r=-0.13, P<0.05) has a significant correlation. In correlation analysis of elements and the number of births, the Mg (r=0.14, P<0.05) and Pb (r=0.12, P<0.05) were significantly correlated. Survey of the content of serum Mg of miscarriage was 3.29+0.32 mg/L, no abortion was 3.33+0.37 mg/L, the difference was statistically significant (P<0.05). Investigation of serum Cr in patients with breast disease (1.91+0.53 g/L) and with no group (1.62+0.61 g/L) the difference was statistically significant (P<0.05). Suffering from uterine diseases survey in serum Mg, Cu, Cr, Mn content (3.16+0.32 mg/L, 1.01+0.21 mg/L,1.81+0.53 g/L,+2.34 2.09 g/L) and did not suffer from uterine disease group (3.31+0.37 mg/L,1.16+0.34 mg/L,1.63+0.61 g/L,3.43 +2.31 g/L) significantly different.Conclusions1?To verify the flame atomic absorption spectrophotometric method determination of serum calcium, copper, magnesium, iron, zinc content of five elements, analysis of the detection limits, precision (including intra day and inter day precision), relative error, and table recovery, the results show that this method can meet the requirements of determination.2?To verify ICP-MS determination of serum boron, chromium, manganese, cobalt, nickel, arsenic, selenium, cadmium and lead total nine elements content, the results show that this method can meet the requirements of determination.3?The content of serum elements have no significant correlation of research target with menstruation or not, menstrual menarche age, menstrual cycle days, menses days, taking birth control pills, whether patients with pelvic inflammatory disease or vaginitis. (P>0.05)4?The content of Clear Boron element has a protective effect on the regularity of the menstrual cycle. For women with irregular menstruation appropriate supplement of Boron elements, can be regarded as a promotion measures.5?The content of chromium and selenium in serum has a protective effect on reducing dysmenorrhea The appropriate supplement of chromium and selenium in women with dysmenorrhea can be regarded as a promotion measure.6?The content of arsenic in serum has a induced effect on the menopause of respondent.The physician can guide or treatment of menopause or menopause symptoms occur in women, arsenic content in blood, can be regarded as a reference index of the diagnosis and treatment.7?The contents of chromium and nickel in serum of the subjects are related to the number of pregnancies. For women with pregnancy disabilities can be detected in the blood of chromium and nickel elements, to provide a reference indicators for the physician's diagnosis and treatment.8?The contents of magnesium and lead in serum are related to the number of birth. The physician can detect the content of magnesium and lead in blood of the women with fertility problems, which can be regarded as a reference index for diagnosis and treatment.9?The content of magnesium in serum has inhibitory effect on the number of abortion. Magnesium can be considered as a protective element to reduce the occurrence of abortion.To detect the content of magnesium in the blood, which can be regarded as the reference index for doctors to judge, make a diagnosis and give treatment. Recommendations for women in pregnancy and pregnancy may be appropriate to add some.10?The content of chromium in serum has the effect on the risk of breast disease. The amount of chromium content in serum, as a reference index for judging the risk of breast disease, early detection and early prevention, early treatment, to provide some data support.11?Serum magnesium, copper and manganese have a protective effect on the risk of uterine disease, and the content of chromium has a role in the risk of uterine disease. Magnesium, copper and manganese can be regarded as a protective element in the uterus, the amount of serum chromium content, as a reference index for judging the risk of uterine disease, early detection and early prevention, early treatment, to provide some data support. |
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