Clinical And 1H-NMR-based Metabonomics Study On Mechanism Of Tongfuxiere Decoction Treatment In Hypertensive Intracerebral Hemorrhage

ObjectivesTo study the changes of serum metabolites in phlegm and blood stasis of hypertensive intracerebral hemorrhage from the perspective of metabolomics,to find the relevant pathologic markers of secondary brain damage,to explore its pathological mechanism and to study the effects of Tongfu Xiexue Huoxue Decoction on promoting hypertension The possible mechanism of the diagnosis of brain damage by phlegm and blood stasis after cerebral hemorrhage is the basis for the clinical application of Chinese medicine.MethodsOur study included 12 patients who underwent surgical treatment from hypertensive intracerebral hemorrhage from 2015-7-1 to 2016-9 months.The 12 blood pressure(1H-NMR)CPMG pulse sequences were used to detect the 12 hypertension Cerebral hemorrhage patients before surgery,and postoperative 1,3,7,12 days plasma samples,each patient collected five plasma samples,the experiment collected a total of 60 blood samples,all patients before surgery,postoperative 1 Day,postoperative day 3,postoperative day 7 and postoperative day 12 were divided into 5 groups.All patients were treated with Tongfu diarrhea.The changes of metabolites content in different groups were analyzed by principal components analysis(PCA)and PLS-Discriminant Analysis(PLSDA).The nuclear magnetic resonance spectra of plasma and the corresponding metabolites The changes of metabolites in the different groups were analyzed by statistical paired t test,and the changes of metabolites in different groups were compared with the metabolic level.The effects of Tongfu Xiehuoxuai mixture on hypertensive intracerebral hemorrhage The mechanism of postoperative injury repair,and to explore the essence of phlegm and stasis syndrome after hypertensive intracerebral hemorrhage.ResultIn the clinical trial,the plasma metabolites of the five groups were detected by 1H-NMR,and the patients were divided into two groups:preoperative,postoperative day 1,postoperative day 3,postoperative day 7 and postoperative day 12(P<0.05).Pattern recognition and principal component analysis(PCA)showed that the formation of phlegm and blood stasis at 3 days after hypertensive intracerebral hemorrhage was differentiated before and after taking Tongfu Xiehe mixture.There were 6 specific metabolites in the plasma of the group before and 1 day after operation:alanine,isoleucine,a-ketoglutarate,glycoprotein,prostaglandin,very low density lipoprotein The There were 6 specific metabolites in the plasma of the patients in the first day and the third postoperative day.6:γ-aminobutyric acid,a-ketoglutarate,betaine,serine,prostaglandin El,Lipoprotein;the third day of the postoperative group and the 12th day after the group of patients with different plasma specific metabolites 6:N-acetyl glycoprotein,a-glucose,choline,inositol,bright Amino acid,glutamine.There were two specific metabolites in the plasma of the 7th day and the 12th day after operation:2:N-acetylglucosamine and lactic acid.ConclusionAfter hypertensive intracerebral hemorrhage,the brain tissue is in a high energy consumption state,gluconeogenesis,fatty acid,tricarboxylic acid circulation disorder,immune function,excitatory amino acid neurotoxicity.When the hypertensive intracerebral hemorrhage surgery phlegm stasis syndrome formation,taking Tongfu Xiexue Huoxue Tang can improve the body’s brain after the fat,glucose metabolism,reduce ischemia and hypoxia damage,stabilize the cell membrane,maintain cell structure integrity,Damage the cell repair,maximize the recovery of nerve cell function,improve the prognosis.After the use of traditional Chinese medicine,γ-aminobutyric acid(GABA)can release a large number of anti-glutamate and other excitatory amino acid neurotoxicity,to complete the protection of neurons,Tongfu Xiexue Huoxue Chinese medicine can maintain neuronal energy metabolism steady state Promote the survival of neurons.