Cold-induced RNA-binding Protein Expression And Clinical Significance In Patients With Chronic Heart Failure

Objective: Chronic heart failure(Chronic heart failure, CHF) is the final destination of the various efforts of the disease, and its pathogenesis involves in inflammation, myocardial apoptosis, myocardial fibrosis extracellular matrix and so on.After long-term efforts, awareness of chronic heart failure therapy has evolved from short-term hemodynamics and pharmacological measures to restoration of long-term strategy. The goal is not only to treat heart failure, alleviate symptoms and improve quality of life, but also to remolel the pathogenesis of myocardial, reversals and slow down the deterioration of cardiac remodeling,improve long-term prognosis of patients with heart failure and reduce mortality.Despite the continuous improvement of HF therapy in the past 20 years, but the5-year mortality rate is still up to 45-50%. So we urgently need to be study the pathogenesis of HF further understanding, and timely to find better treatment to save patients with HF. CIRP(cold inducible RNA-binding protein) is a protein of recent research focus.Numerous studies have shown that it has cytoprotective effects, at low temperatures, hypoxia and ultraviolet irradiation condition can be upregulated.And it indicates CIRP expressed constitutively in the heart. CIRP can regulate circadian rhythm effects in myocardial cells, but also to restore function after myocardial infarction. CIRP regulate the expression and function of cardiac repolarization by controlling the potassium channels(Ito) in myocardial cells.Therefore, we speculate that CIRP may play an important role in the pathogenesis of chronic heart failure. To this goal, the group collected tissue samples in patients and dectected the m RNA of CIRP by real-time PCR and protein expression of CIRP by immunohistochemistry, respectively.Methods: 10 cases of open heart valve replacement surgery were divided into the experimental group(patients with chronic heart) five cases and the control group (patients without heart failure) 5 cases, right atrial appendage tissue was taken during surgery. CIRP expression was accessed by real-time PCR and CIRP expression and distribution were studied through immunohistochemical stainin.Result:1. The clinical data analysis showed that the EF(%) value level(see Table 3.2) in the experimental group was lower than those in the control group; C-reactive protein(hs CRP) and BNP or Pro-BNP were higher than those in the control group.2. RT-PCR showed that compared to the control group, CIRP m RNA expression in the experimental group was significantly reduced and the difference were statistically significant P <0.05;3. Immunohistochemical staining analysis demonstrated that: Compared to the control group, CIRP m RNA expression in the experimental group was significantly down- regulated and the differences were statistically significant P <0.05.Conclusion:1. CIRP expression in chronic heart failure group was significantly downregulated.2. This study suggests CIRP have some relevance in chronic heart failure, and it may play a role in the pathogenesis of heart failure.