The Kidney And Spleen,method Of Detoxification,Pharynx IgA Nephropathy Model In Rats The Effect Of IL-2,IL-10

Purpose: Through the experiment to observe the kidney and spleen,method of detoxification,pharynx IgA nephropathy model in rats the effect of IL-2,IL-10,Further research to explore the kidney and spleen,detoxification,pharynx mechanism method,the curative effect of treatment of IgA nephropathy,Through a lot of experimental data,provide scientific basis for the clinical treatment of IgA nephropathy.Method:Selected 80 healthy male clean level of Wistar rats.weight(200 +)20 g,Using the method of stratified random adaptability will have been feeding 1 week 10 rats were divided into normal group,made 70 module.In order to get the IgA nephropathy rats model,We give oral lavage building modules bovine serum albumin,lipopolysaccharide tail vein injection of 0.25 g/L and subcutaneous injection of 25% carbon tetrachloride,and Give the blank group rats saline solution.Again the randomized method,will verify successful module were randomly divided into model group,kidney and spleen,detoxification,pharynx Chinese medicine high,medium and low dose group,tripterygium wilfordii glycoside.To normal saline lavage blank group and model group every day,each 4 ml.Gives the kidney and spleen,detoxification,pharynx Chinese medicine high,medium and low dose group of 4ml/(kg,d)feeding,content of 32 g/kg respectively,16 g/kg,8 g/kg.For 8 weeks.During this period,observing the status of daily life of a rat,regularly test between groups in the rat liver function,renal function and serum 24-hour urine red blood cell count and 24 hours urinary protein quantitative change.By enzyme-linked immunosorbent determination of IL-2,IL-10 in the tissue of the kidney.Using fluorescence immunoassay method for determining IgA complex deposition in the kidney tissues by immunohistochemical method to observe IL-2,IL-10 orientation distribution.ResultIgA nephropathy rats model successfully,Completed in accordance with the relevant provisions to econometric model group,positive control group and treatment group take medicine.Experiments,a total of 22 rats death,Including testing is successfully executed death 10 rats.During the building modules.Building modules eight rats dead?When feeding died four rats,Including 4 rats of glucoside tripterygium wilfordii more than three positivecontrol group,low dose treatment group 1 only.In the whole experiment process,Blank group rats did not see unusual,live in good condition.Build module 70 rats after subcutaneous injection of 25% carbon tetrachloride,spiritual malaise,lose luster of hair,diarrhea,weight decreased abnormal performance,etc.After a period of treatment,the whole body symptoms improved,ease the symptoms.Through the detection module in the rat urine red blood cell count and urine protein quantitative increase,Blank group was no hematuria proteinuria,Glucoside tripterygium wilfordii more than the control group and the kidney and spleen,detoxification,pharynx Chinese medicine high,medium and low dose treatment group rats decreased.(P<0.05),Compared the is no significant difference between groups?(P>0.05)?Conclusion:(1)through the view of Liu Huiyu,With carbon tetrachloride,lipopolysaccharide and bovine serum albumin these drugs can successfully construct IgA nephropathy animal experimental model.(2)Both tripterygium wilfordii glycoside,and kidney and spleen,detoxification,pharynx Chinese traditional medicine can reduce the model rat red blood cell count 24 hours and 24 hours urinary albumin excretion rate.(3)Experimental data show that no statistical differences between groups in the rat liver,kidney,But glucoside tripterygium wilfordii more than control group in the rat liver damage is more than using tripterygium wilfordii glycoside has certain harm to liver.(4)Tripterygium wilfordii glycosides and kidney and spleen,detoxification,pharynx Chinese medicine in the treatment of IgA nephropathy hematuria proteinuria side effect is the same.(5)Each treatment group comparison,kidney and spleen,detoxification,pharynx is probably the formulas by adjusting the IgA nephropathy model rat renal tissues of cytokines IL-2 and IL-10 levels,to slow the progress of the disease.(6)Comparison model group and blank group,the IL-2 of kidney tissue express to reduce,and IL-10 rise.By tripterygium glycosides more control group and the kidney and spleen,detoxification,pharynx after dosing.The level of IL-2 ascent,and the IL-10 down.After extrapolated,the abnormal expression of IL-2 and IL-10 is the cause of disease.