The Expression Of Hepatocyte Growth Factor Level During Orthodontic Force In Gingival Crevicular Fluid

Objective:To evaluate the dynamic changes of hepatocyte growth factor (HGF) in gingival crevicular fluid(GCF)during orthodontic tooth movement by using enzyme-linked immunosorbent assay method and investigate the biochemical process of the periodontal remodeling during orthodontic tooth movement to provide reference for clinical value to choose the appropriate force.Methods:1. Sixteen patients whose first premolar were required to extract were choosen as subjects.The patients included seven males, nine females, and their age was from twelve to eighteen years old, who installed fixed appliance. Cases were randomly divided into two groups, eight cases in each group.2.Straight wire appliance was applied on the teeth and aligned the teeth.In each patient, the right maxillary canine was received orthodontic force of 100g?250g from mesial to distal, while the left maxillary canine was served as control without any force. Subsequently,we performed the ELISA to test the HGF by collecting the gingival crevicular fluid of each group(both test groups and control groups) 0 day before concentration force and 1?3?7?14 days after exerting.3.GCF collection and measurement:GCF was collected by 30# filter paper. During GCF sample collection, we isolated the tooth by contton and inserted the 30# filter paper gently gingival sulcus until you felt a mild resistance at distal side of each canine.Nextly,we kept it in the gingival sulcus for a minute, then put it in the EP tube immediately and recorded the periodontal clinical parameters. If the filter paper was contaminated with blood, it would be discarded immediately. EP tube was weighed and then put into-80? refrigerator. The concentrations of HGF were determined using human HGF ELISA kit. The assay was carried out according to the manufacturer's instruction.4.The concentrations of HGF in GCF were exermined by the ELISA technique following manufacturer's recommendations. We analyzed the data with statistical method.Results:1.The periodontal clinical parameters were shown no significance before and after application of force(P>0.05).2.Detecting the weight of GCF before and after in different force:for 100g group:the weight of GCF increased at 1 d,3d, and the statistical analysis turned out that the weight of GCF was significant different at 3d. For 250g group:the weight of GCF increased at 1d,3d, declined at 7d, and the statistical analysis turned out that the weight of GCF was significant different at 3d,7d.3.The alteration of concentration of HGF in GCF from 100g group:it raised gradually at 1 d, 3d, after exerting the force, declined at 7d, returned to the level before the force at 14d. The change of concentration of HGF in GCF from 250g group:it raised gradually at 1 d,3d, after exerting the force, reached the peak at 7d, after 7d, returned to the level before the force at more than 14d.The concentration level of HGF in treatment and control group was significant different(P<0.05).Conclusions:1.HGF is detectable in gingival crevicular fluid under healthy state.2.The expression of HGF in GCF was increased under orthodontic force,indicating that HGF was involved in periodontal remodeling during tooth movement induced by mechanical force.3. Appropriate orthodontic force could lead to periodontal tissue of normal physiological alterations. On the contrary, the excessive orthodontic force could cause periodontal tissue damage, which affected orthodontic treatment.