| Objective:The aim of this study was to examine the possible association between single nucleotide polymorphisms of +3954C/T in IL-1B gene?-Taq I T/C in VDR gene and-1607 1G/2G in MMP-1 gene and chronic periodontitis susceptibility and severity of chronic periodontitis and severe chronic periodontitis susceptibility in Wa and Han population of Gengma, which is in Lincang city Yunnan province, and to provide basic data of the distribution of susceptible genes to chronic periodontitis in Wa and Han population of Gengma.Methods:A total of 285 subjects were enrolled in this study (including155Wa subjects with chronic periodontitis and 48 periodontally heathy subjects,61 Han subjects with chronic periodontitis and 21 periodontally heathy subjects). All the subjects received a periodontal examination including full-mouth probing depths, clinical attachment loss measurements and bleeding on probing.Periodoontal healthy groups and chronic periodontitis groups formed according to periodontal conditions were healthy or not, further more, three groups formed according to periodontal conditions were slight, moderate and severe periodontitis groups. Genomic DNA was obtained from the buccal swabs with the buccal mucosal epithelial cells of the subjects, IL-1B+3954 (C/T). VDR-Taq I (T/C) and MMP-1-1607 (1G/2G) gene polymorphisms were genotyped using Sanger DNA sequencing method. SPSS 17.0 software was used to analyze the differences of the frequencies of 3 polymorphisms among groups.Results:1 Analysis of polymorphisms showed no differences in distribution of the +3954 C/T variants in the IL-1B gene between Wa and Han population, the same to the healthy and chronic periodontitis groups; The data obtained revealed no differences among the slight, moderate, severe chronic periodontitis groups and healthy groups, also no differences between the healthy+slight+moderate groups and severe chronic periodontitis groups (P>0.05).2 Analysis of polymorphisms showed no differences in distribution of the -Taq I T/C variants in the VDR gene between Wa and Han population. For Wa population, the data obtained revealed no differences between the healthy and chronic periodontitis group and no differences among the slight, moderate, severe chronic periodontitis group and healthy group, no differences between the healthy+slight+moderate group and severe chronic periodontitis group (P>0.05) as well. For Han population,there were no differences in distribution of the-Taq I genetypes in the VDR gene between the healthy and chronic periodontitis group, and no differences among the slight, moderate, severe chronic periodontitis group and healthy group, also no differences between the healthy+slight+moderate group and severe chronic periodontitis group (P>0.05). However, the multiple logistic regression analysis indicated diffences in distribution of the-Taq I T/C alleles in the VDR gene between the healthy and chronic periodontitis group after correction of age, C allele was significantly more frequent in the periodontitis group. Compared to T allele, C allele was likely to be the protect factor of chronic periodontitis (P=0.015, OR=0.07,95% CI=0.008-0.595).3 Analysis of polymorphisms showed no differences in distribution of the-1607 1G/2G variants in the MMP-1 gene between Wa and Han population. For Wa population, the data obtained revealed no differences between the healthy and chronic periodontitis group, and no differences among the slight, moderate, severe chronic periodontitis group and healthy group; There were no differences between the healthy +slight+moderate group and severe chronic periodontitis group(P>0.05). For Han population, the data of this study didn't find differences in distribution of the-1607 1G/2G variants in the MMP-1 gene among the slight, moderate, severe chronic periodontitis group and healthy group and also no differences between the healthy +slight+moderate group and severe chronic periodontitis group(P>0.05). However,the multiple logistic regression analysis indicated diffences in distribution of the-1607 genotypes in the MMP-1 gene between the healthy and chronic periodontitis group after correction of age, copmpared to 2G/2G genotype,1G/2G and 1G/1G genotypes increased 5 and 7 times susceptibility of chronic periodontitis, respectively (OR=5.431,95%CI=1.799-16.398; OR=7.178,95%CI=1.572-32.784).Meanwhile, the frequency of 2G/2G genotype in Wa and Han healthy subjects were 39.6% and 57.1% respectively, which were higher than both domestic and oversees reports.Conclusions:1 Based on the results of this study, no significant association is found for the +3954 SNP in IL-1B gene with susceptibility of chronic periodontitis or severity of chronic periodontitis and severe chronic periodontitis susceptibility in these Wa and Han population.2 Based on the results of this study, no significant association is found for the-Taq I SNP in VDR gene with susceptibility of chronic periodontitis or severity of chronic periodontitis and severe chronic periodontitis susceptibility in these Wa population.No significant association is found for the-Taq I genetypes in VDR gene with susceptibility of chronic periodontitis or severity of chronic periodontitis and severe chronic periodontitis susceptibility in these Han population, while C allele may be the protect factor in Han population.3 Based on the results of this study, no significant association is found for the -1607 SNP in MMP-1 gene with susceptibility of chronic periodontitis or severity of chronic periodontitis and severe chronic periodontitis susceptibility in these Wa population.No significant association is found for the-1607 alleles in VDR gene with susceptibility of chronic periodontitis or severity of chronic periodontitis and severe chronic periodontitis susceptibility in these Han population, while the genetypes may associate with susceptibility of chronic periodontitis in Han population.4 Based on the results of this study, we find the frequency of 2G/2G genotype in Wa and Han healthy subjects are higher than both domestic and oversees reports. |
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