Study On Safety And Therapeutic Efficiency Of Liposome-encapsulated Hemoglobin For Acute Hemorrhagic Anemia In Dog

In the pet in the clinical diagnosis and treatment,when the dog because of anemia critical to life,often through blood transfusion therapy for first aid.Is the most common blood transfusion of whole blood,sometimes according to condition the need for component blood transfusion.Because the dog blood type,blood for type and crossmatch before the clinical blood transfusion,especially for the second time of more than dogs.Cross matching test process complicated and tedious,affect the efficiency of emergency,to that end,blood substitutes have developed for clinical trials.Hemoglobin liposomes(Liposome encapsulated hemoglobin,LHb)is through the artificial preparation of Liposome embedding hemoglobin,made of a kind of biological products,also known as "red",no antigenicity.In this paper,by adjusting the proportion of reagents,and discusses the embedding rate of high and low,at the same time the LHb infusion into healthy dogs and uncontrolled hemorrhagic anemia,observe the feasibility and the anemia,improve effect,clinical research and application for pet provide guidance.Experiment 1.Preparation of liposome-encapsulated hemoglobin(LHb)of cannineThe experimental conditions in the laboratory,adopts rotary evaporation combining ultrasonic oscillation liposome preparation of hemoglobin,main reagent is soybean lecithin,cholesterol,vitamin E and chloroform.A fixed amount of vitamin E,design different soybean lecithin,cholesterol than quality,discusses the influence of the embedding rate of hemoglobin,and determine the embedding rate is the highest hemoglobin liposome characteristics,testing the stability of its in vitro from several aspects.Results show that when the quality of soybean lecithin and cholesterol than 2:1,the embedding rate is highest,at 37.64%.Evaluate the blood gas in the LHb suspension,also confirmed that the preparation of artificial red blood cells with oxygen affinity ability.The LHb microscope particle diameter between 4.6-7.9 ?m.Byobserving the appearance of the LHb color changes,oxygen permeability and affinity,found the LHb in 4? to save time in a month or so.Experiment 2.Safety of LHb for canine in clinical testThe experimental research on hemoglobin liposomes in the clinical safety of dogs.Select 12 healthy adult dogs,randomly divided into 2 groups,each group of 6,number of A and B,A set of 15 mL per dog input ringer's solution,group B every dog input equivalent LHb suspension,strengthen inject 1 times A week to 6 weeks,and record the test,4,6,8.2 weeks observation clinical symptoms between groups of dogs,detection of blood routine,blood biochemistry,routine urine index changes.The body before the trial,preparation of first LHb matches with whole blood group B dog,observe whether the erythrocyte agglutination or hemolysis reaction.The results showed that:in vitro matches not present erythrocyte agglutination test or hemolytic reaction.Clinical abnormal short LHb group,part of the testing index,abnormal non-pathological other compared with group A test,the difference was not significant.By means of a dog vital signs were observed,blood routine,blood biochemistry,routine urine test,liposome preliminary confirmed that the hemoglobin of dog clinical safety.Experiment 3.The therapeutic effect of liposome-encapsulated hemoglobin for acute hemorrhagic anemia in dogsIn order to observe the therapeutic effect of artificial liposome-encapsulated hemoglobin(LHb)for canine with acute hemorrhgic anemia,12 dogs with hemorrhagic anemia were divided into four groups,and 3 dogs in each group:no treatment group(Sham),liposome encapsulated hemoglobin group(LHb),shed autologous blood group(SAB),and saline group(Saline).Clinical symptoms were observed and blood routine,blood biochemistry and urine routine indices were measured.As a result,the anemia symptons were improved obviously in LHb and SAB when compared with the Sham and Saline groups;hemoglobin concentrantion on the first day(167.67±2.96 g/L)was higher than pre-blood loss(135.33±3.18 g/L),no significant difference(P>0.05)was found between LHb and SAB from the third day.Changs of leukocyte(WBC),neutrophilic granulocyte(Gran),monocyte(Mono)were similar trends,and significant difference(P<0.05)was found between LHb and SAB group on first two days;serum total bilirubin was increased and exceeded the interval reference on the first two days after LHb infusion,significant difference(P<0.05)was found between LHb and SAB from first day to fourth day;marked increase in urine indicators and continued four to five days.It is showed that LHb as a blood substitutes for anemia treatment has obvious effect.