Study On Treatment Of Traditional Chinese Medicine Compound Preparation On Kidney Injury Of Chicken

The urate deposition of chicken is a metabolic disease that caused by kidney damage and kidney function decline,thereby causing uric acid excretion blocked,formatting uric acid poisoning due to various reasons.There are no specific drugs on treatment for complicated etiology.Traditional Chinese medicine compound preparation has a long history and some drugs have a mild effect on the treatment of kidney disease,it also can restore the renal function,and there is no damage on the spleen and stomach.Traditional Chinese medicine compound preparation which the experimental selected has the function of renal antiasthmatic,clearing damp and promoting diuresis permeability and rapid recovery of renal function.The experiment proved a good clinical effect.But the effect of traditional Chinese medicine compound preparation for renal urate in chicken from the chicken kidney damage and recovery mechanism was unclear.The aim is to explore the excretion effect of urate deposition and the recovery effect of the chicken kidney damage through treating chicken model of hyperuricemia by compound traditional Chinese medicine preparation.Methods:?1?Pathological Model of renal injury in chicken was constructed by feeding high calcium and high protein feed.Then,all of experiment chicken was randomly divided into control group,model control group,low-dose and high-dose treatment group.Each group of chickens were weighed and necropsied to observe the recovery effect of traditional Chinese medicine compound preparation on chicken before and after the experiment,and the blood was collected to detect the biochemical indexes during the experiment.The kidney tissue of chicken were selected to make pathological section to observe and the variation of renal pathological tissue.?2?The experimental chickens were randomly divided into 4 groups,and that were treated with 3,5 and 10 times of clinical dosage,and the eating situations and toxic reactions were recorded during the treatment,and the chickens were autopsied and counted the organ index at 0th,7th and 14 th of the experiment.At the same time,KM mice was gavage administration with different concentration to measure median lethal dose(LD₅₀).If the mice did not appear death,then the KM mice was gavage administration with 2 g/mL concentration three times a day to determine the maximum tolerated dosage.The situation of poisoning and death was observed during the experiment.Results:?1?The high-dose treatment group had no death and the mortality in low-dose group was significantly reduced after treatment,compared with the model control group,the level of serum uric acid,aspartate aminotransferase?AST?and kidney coefficient in the treatment group was significantly lower than that in the control group?P<0.05?.The renal pathological tissue slices showed that the glomerular swelling was significantly restored,and the structure of renal tubular tissue was complete,the interstitial tissue had no fibrosis in the treatment group.?2?The experimental chicken had no abnormal reaction after treatment.After a week,the viscera had no obvious lesions and the difference of internal organs index among 4 groups was not significant.The experiment mice had no significant abnormal reaction,and no death,so the LD50 of mice could not be measured.The result of the maximum tolerated dosage indicated that according to maximum to dose three times a day,a total of daily dose was 120 g and the mice had no symptoms of poisoning and did not appear death.Conclusion: Traditional Chinese medicine compound preparation could promote the excretion of uric acid salts and had a certain effect on kidney damage caused by high calcium and high protein.