| Avian gout is a prevalent disease, especially for visceral gout, and it often results in severe loss of production of fowl. Many factors could induce avian gout and diet with high calcium and/or protein is the most common factor in our country.The abnormal metabolizability of uric acid and turbulence of acid-base balance and electrolyte have a consanguineous connection with mechanism of avian gout. In this experiment, effects of diet with high calcium and/or protein on function of liver and kidney and acid-base balance of pullets were studied, and the aggradation mechanism of urate was also studied in vitro in order to discuss ulteriorly the mechanism of avian gout induced by diet with high calcium and/or protein.In experiment 1, effects of the three kinds of diets with high calcium, high calcium and high protein or high protem on the dynamic changes of some biochemical parameters in blood of pullets were observed. One hundred and sixty pullets were divided randomly into four groups at 35 days of age. A diet with normal calcium (1.00%) and normal protein (17.53%) was fed to control pullets. Other groups were fed a diet (HC) with high calcium (3.78%) and normal protein (17.42%), a diet (HCHP) with high calcium (3.78%) and high protein (24.25%) and a diet (HP) with normal calcium (1.00%) and high protein (24.52%) respectively.The experiment last for 32 days, In order to determine involved biochemical Parameters, blood was collected respectively at the pre-experiment and 8th, 16th , 24th, 32th day of the experiment and serum was separated. The results showed that the HC\ HCHP and HP diet could significantly increase serum AST , ALT,ALP and GGT (P<0.05 or P<0.01) activities of pullets, the HC diet could increase serum TB and CB levels (P<0.05 or P<0.01) . It can be concluded that all the three diets could result in damage of liver of pullets. The HC and HCHP diet could significantly increase serum Ca and UA levels and the HCHP and HP diet could significantly increase serum BUN and Cr. |
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