Crataegus Pinnatifida Bge Chemical Composition And Quality Specification Research

Fructus crataegi is the rose family (Rosaceae) plant Crataegus pinnatifida Bge.or Crataegus pinnatifida Bge. var. major N. E. Br. dry mature fruit.Also names the red fingernail solid、the red date、the hill fruit、Mao Cha、the monkey stubble、the azalea fruit、the Tang pear、the camellia、the acid to look up.Taste acid、Gan, micro warm, turns over to the spleen、the liver、the kidney to pass through, has the digestion to be good for the stomach, dispels symptoms such as bloating disperses merit of the stasis. "The fructus crataegi pinnatifidae" the host produces in provinces and so on Liaoning、Shandong、Henan、Hebei, take Liaoning、Shandong producing as a place.Carries on preliminary study to fructus crataegi chemical composition, through gathers the amide chromatograph、the silica gel column chromatography、the gelatin column chromatography and so on heavy crystallization, obtains 4 triterpenoids kind of compound from 70% ethyl alcohol extraction separation,3 flavonoids,2 steroids compounds, through methods and so on physics and chemistry nature and spectrum data analysis have appraised these 9 compounds, they respectively be lupeol (1), a-amyrin (2), ursolic acid (3), oleanolic acid (4), hyperoside (5), rutin (6), kaempferol (7),β-sitosterol (8), daucosterol (9).lupeol (1), a-amyrin (2) is the separation obtains for the first time from this plant.To the fructus crataegi raw material for medicine plant shape、raw material for medicine character、micro、aspects and so on carry on the distinction, has established in for the first time the RP-HPLC method determination different habitat fructus crataegi the lupeol content method, and has established in the RP-HPLC method determination different habitat fructus crataegi hyperoside content method, has conducted the research to the fructus crataegi raw material for medicine highly effective liquid chromatography fingerprint atlas.Has established in for the first time the HPLC method determination fructus crataegi’s fruits、the kernel、the leaves、branchs the lupeol content method, and has established in the HPLC method determination fructus crataegi’s fruits、the kernel、the leaves、branchs the fructus crataegi hyperoside content method.To the fructus crataegi pinnatifidae’s two variety Crataegus pinnatifida Bge.and Crataegus pinnatifida Bge. var. major N. E. Br. plant shape、the character、micro、aspects and so on carry on quite distinguish, has established in for the first time the RP-HPLC method determination Crataegus pinnatifida Bge.and Crataegus pinnatifida Bge. var. major N. E. Br. the lupeol content method, and has established in the RP-HPLC method determination Crataegus pinnatifida Bge.and Crataegus pinnatifida Bge. var. major N. E. Br. hyperoside content method.This article findings have laid the foundation for quality specification’s further research, has provided the scientific basis for the fructus crataegi raw material for medicine’s quality appraisal and quality specification’s establishment.