The Yiqi Mixture On The Immune Inhibition Effect Of Immune Function In Rats

ObjectiveImmunosuppressed rat model. Determination of model rats serum IgG, IgA, IgM content and the content of IL-2, thymus index and spleen index immune indexes and Sijunzi decoction as control, evaluation of Yiqi mixture immune regulatory role.Methods44 rats were randomly grouped, divided into blank group, model control group, yiqi mixture of high, medium and low dose group, sijunzi decoction, the control group and the blank group, model group and each group of ten, the rest of the group 6 in each group.Blank group and model group was given saline(3 ml/day) to fill the stomach;Yiqi mixture high, medium and low dose group were given 12.6 g˙ kg-1, 6.3 g˙ kg-1 and 3.2 g˙ kg-1 yiqi decoction lavage mixture of water, ˙ drug control group to give 5.5 g˙ kg-1 soup sijunzi decoction lavage;Except the blank group, the rest of the group 3 d before lavage, after dosing 1 h, give the intraperitoneal injection of cyclophosphamide 50 g˙ kg-1 building.After the completion of the building, on the seventh day of experiment, respectively from the blank group and model group randomly take out four rats, male and female half, said rat body weight, profile control in the thymus and spleen, thymus index and spleen index, indicators of success to make mould.Take the blood on the 14 th day, abdominal aorta, centrifugal in serum, using ELISA method for determining the content of IL- 2, with automatic biochemical analyzer(transmission turbidimetry) for determining the content of IgG, IgM and IgA, profile control in rat thymus and spleen, thymus index and spleen index calculation.Results:1. On the 7th day, modeling success indicators to determine. General: model group have varying degrees of diarrhea, even anal off, hair color dull yellow, hair get together to erect, less activity, group poly Baotuan, listlessness, loss of appetite. The model index: Determination of rat spleen index, thymus index and weight. The results showed that, compared with the blank group, model group and the spleen index and thymus index decreased significantly(P < 0.01) and body weight of the rats in the model group reduced(P < 0.05); indicating the modeling was successful.2. Rats in the model group, the serum IgM, IgA, IgG, IL-2 content compared with the blank group was significantly lower(P < 0.05); Yiqi mixture after treatment, serum IgM, IgA, IgG, the content of IL-2 and spleen index, thymus index compared with the model group increased significantly(P < 0.05); and with high Yiqi mixture, middle dose group effect significantly.Conclusion:1、low immune function can lead to IgG, IgA, IgM, IL-2 content in the body is reduced; can make the spleen, thymus atrophy, leading to the storage of T, B cell content decreased, thereby inhibiting the body immune function.2、Yi Qi mixture through increasing IgG, IgA, IgM, IL-2 content, increase the spleen index and thymus index, and play the role of immune regulation.3、the improvement of IgG, IgA, IgM,, IL-2, spleen index and thymus index, spleen index and thymus index of the ability, compared with four gentleman decoction has certain advantages, and its dose effect relationship and mechanism of action, need to do further research.