Yiqi Complex Mixture Of Arteries And Veins Contracts Before Sexual Period Treatment Mechanism Of Experimental Research

The pathogenesis of premature ventricular beats(PVBs) is that ventricular with unusual self-discipline because of pathological changes, and when self-discipline absolutely or relatively exceeds the sinus node, they will be issued ectopic impulse, instead of controlling the activities of the heart, thus, generated the PVBs. Antiarrhythmic drugs affect myocardial cell membrane ion channels, changing the ion flow, thereby changing the electrophysiological characteristics of myocardial cells, and inhibiting the self-discipline of ectopic pacemaker, terminating of reentry, inhibiting depolarization and triggered excitement, thus play a role in the antiarrhythmic effect. While western antiarrhythmic drugs are almost exist potential arrhythmogenic effect. So there are some limitations in the treatment.Chinese medicine shows certain advantages in the treatment of PVBs. Previously literatures report that single herb has different mechanisms of antiarrhythmic. The clinical palpitations patients are more with deficiency mixed syndromes. Its pathogenesis is mostly Qi deficiency and blood stasis, phlegm and blood stasis. Yiqifumai mixture has effect of replenishing Qi and vivid the blood, dispelling phlegm and calm senses, and has effective clinical applications. Therefore, the study is intended to evaluate the overall efficacy of antiarrhythmic of Yiqifumai mixture, and explain the mechanism of the anti-arrhythmic, in order to provide more complete and reliable support for the clinical treatment.PurposeIt uses the classic models of aconitine, calcium chloride and isoproterenol to cause arrhythmias, and the small animals ECG telemetry technology, to evaluate the overall efficacy of Yiqifumai mixture on PVBs. It uses the patch-clamp technique, recording the impact of the action potential on cardiac myocytes. Furthermore, it describes the mechanisms of antiarrhythmic effect, in order to provide more complete and reliable support for the clinical treatment.MethodUsing aconitine, calcium chloride arrhythmogenic models and the SD rats are randomly divided into wihte type group (WT), Fulvtongmai mixture (low, medium, high dose) groups (LOW, MID, HIGH). Pumping the aconitine solution or calcium choride solution from the femoral vein in a constant speed, and record the time when PVBs, ventricular tachycardia (VT), ventricular fibrillation (VF) and cardiac arrest (CA) and arrhythmia appear. Calculating the incidence of PVBs, VT, VF and the dosage of aconitine and calcium chloride. Using isoprenaiine (Iso)-induced arrhythmia model on spontaneously hypertensive rats (SHR), and using ECG telemetry, recording whether there has single PVBs (SP), paired PVBs (PP), VT in WT group and Chinese Medicine group (CM), and statistic on the incidence of SPR, PP, VT and the numbers and the time of occurrence. They all use to evaluate the antiarrhythmic effects of Yiqifumai mixture. Using the collagenase method in acutely isolated rat left ventricular myocytes, and using patch clamp techique, to record the action potential of ventricular myocytes, in order to explore the antiarrhythmic mechanism of Yiqifumai mixture.Result1. Compared with the WT group, Every dose of CM groups can significantly delay the aconitine induced PVBs, VT and VF time (P<0.05, P<0.01, P<0.01), and significantly increased the aconitine dosage when VT appears(P<0.05, P<0.01, P<0.01); the MID and the HIGH groups also can significantly delay CA time (P<0.01), and significantly increase the dose of aconitine when PVBs, VT, VF and CA appear (P<0.01). Compared with the LOW group, the MID and the HIGH groups can significantly delay PVBs, VT and VF time (P<0.01), and can significanly delay CA time (P<0.05, P<0.01), and significantly increase the dose of aconitine when PVBs, VT, VF and CA appear (P<0.01). It suggests that Yiqifumai mixture has anti-arrhythmogenic effect of aconitine and the effect of the MID and the HIGH dose is much better.2. Every dose of CM groups can reduce the incidence of PVBs, induced by Calcium chloride; the MID and the HIGH groups can reduce the incidence of VF, but there are no significant differences (P>0.05). Compared with the WT group, every dose of CM groups can delay CA time (P<0.01), and can increase the dose of Calcium chloride when CA appears (P<0.05, P<0.01, P<0.01); the MID and the HIGH groups also can significantly delay arrhythmia time (P<0.01), and can significantly increase the dose cf Calcium chloride when arrhythmia appears (P<0.01). Compared with the LOW group, the MID and the HIGH groups can significantly delay arrhythmia time (P<0.01), and can significantly increase the dose of Calcium chloride when arrhythmia appears (P<0.01), and the HIGH group can increase the dose of Calcium chloride when CA appears (P<0.05). It suggests that Yiqifumai mixture has anti-arrhythmogenic effect of calcium chloride and the effect of the MID and the HIGH dose is much better.. 3. The WT group and the CM group have no significant difference in the body weight of rats, heart rate, systolic blood pressure, diastolic blood pressure and mean arterial pressure (P>0.05), the two groups were comparable. After1hour of ECG telemetry, the CM group is lower than the WT group in incidence of SP and VT, but there is no significant difference (P>0.05); the CM group is significantly lower than the WT group in the amount of SP (P<0.05). After2hours of ECG telemetry, the CM group is significantly lower than the WT group in the amount of SP, PP and VT (P<0.05); there has no significant difference in SP time (P>0.05), but the CM group can significantly increase the time of PP and VT (P<0.05). It suggests that Yiqifumai mixture has anti-arrhythmogenic effect of isoprenaline, and the longer, the more significant.4. Compared with the WT group, the CM group has no significant difference in resting potential (RP), overshoot (OS), action potential amplitude (APA) and the maximum rate of rise (Vmax) whether Iso perfusion or not (P>0.05). Before Iso perfusion, compared with the WT group, the CM group can significantly extend APD, APD50and APD90(P<0.05). After Iso perfusion, the WT group and the CM group can significantly extend APD (P<0.01, P<0.05), and the WT group also can significantly extend APD50and APD90(P<0.01). After Iso perfusion, compared with the WT group, the CM group can significantly shortening APD, APD50and APD90(P<0.01). It shows that Yiqifumai mixture can against the prolongation of APD caused by Iso, maybe related to the inhibiton of the late plateau Ca2+current and repolarization K+current.Conclusion1. Yiqifumai mixture has anit-ventricular arrhythmogenic effect of aconitine and calcium chloride. It suggests that Yiqifumai mixture has effect of anit-premature ventricular beats in physiological conditions and it has significant effect in clinical dose (as the dose of the MID group).2. Yiqifumai mixture has effects of anti-ventricular arrhythmogenic effect of isoproterenol. It suggests that Yiqifumai mixture has effect of anti-premature ventricular beats in pathological conditions, and the longer, the more significant.3. Yiqifumai mixture can against the prolongation of APD caused by Iso. It’s mechanism maybe related to the inhibiton of the late plateau Ca2+current and repolarization K+current.