The Study Of Correlation Between Dry Eye Of College Students And Mucin MRNA Expression

Objective 1. To analyze the lifestyle, the eye condition, the prevalence of dry eye and the relevant factors in college students by screening the dry eye condition in one of the Tianjin universities. 2. To compare the changes of mucin m RNA expression in normal and severe dry eye of different genders. 3. To compare the changes of mucin m RNA expression in normal and severe dry eye of same gender.Methods 1. A total of 240 students were recruited from one of the Tianjin universities. The general material data, clinical symptoms and signs were recorded. The students were carried out a series of checks separately: dry eye questionnaire, the uncorrected visual acuity, medical refraction, the anterior segment with slit lamp examination, tear film break-up time, fluorescein staining, topical anesthesia and collect conjunctival cells by conjunctival brush after 15 min. According to Ocular Surface Disease Index, the students were divided into four groups: control group, mild dry eye group, moderate dry eye group and severe dry eye group. The moderate and severe dry eye groups were analyzed further. 2. 20 persons of normal females, 20 persons of normal males, 12 persons of severe dry eye male and 16 persons of severe dry eye female were randomly selected to analyze the mucin m RNA expression by RT-PCR.Results 1. The lifestyle of college students was in a stay up condition. Then college students usually have a high pressure. For them, they need reading more then 10 hours everyday. 22.5% of the students need to use the topic eye drops as asthenopia, and the percentage was as high as 93.1% in the students who need to use the topic eye drops. 2. The spherical equivalent diopter in college students was-3.84±2.26 D. There was no statistical difference in different genders(P=0.413). 49.6% of the students suffered from the low to moderate myopia.3. 14.2% of the students were belong to the severe dry eye and 15.8% of the students were belong to the moderate dry eye. 4. The prevalence of dry eye was 30% in college students. The prevalence of dry eye were 29.7% and 30.1% in males and females respectively. The prevalence of dry eye in males or females almost the same and no statistical difference in it(P=0.317). 5. The lifestyle of the college students has an important influence on the happening of dry eye. The lifestyle of stay up usually induced the percentage up to 55.6%, and compared with other factors, there were statistical differences(p<0.01). 6. The highest incidence of dry eye symptoms were visual fatigue, dryness, itching of eye and blurred vision. Among them, the percentage of visual fatigue and dryness up to 50%. 7. The average of ODSI score was 32.22±8.0. OSDI and TBUT had a negative relationship( P=0.001), but OSDI and fluorescein staining had no relationships(P=0.497). 8. For OSDI score in control and severe dry eye group, there were no statistical difference in different genders(P=0.772, P= 0.334). But the average OSDI score of severe dry eye male group was higher than the female, and the number of people whose symptom rates were greater than or equal to 3 in male were more than female. 9. For mucin m RNA expression in control group, there were no statistical difference in different genders. For severe dry eye group, the MUC2、MUC4 and MUC5 AC m RNA expression had statistical difference in different genders(p=0.030,p=0.000,p=0.017. respectively), and these mucins m RNA expression decreased in male group. 10. For control and severe dry eye group, the MUC1、MUC7and MUC16 m RNA expression had no statistical difference in different genders(P=0.308,P=0.065;P=0.571,P=0.748;P=0.405,P=0.246), and the MUC2 and MUC4 m RNA expression had no statistical difference in females(P=0.549;P=0.229). 11. For male group, there were statistical difference in MUC2、MUC4 and MUC5 AC m RNA expression between the control group and the severe dry eye group(P=0.000, P=0.000, P=0.00. respectively). For female group, there was statistical difference in MUC5 AC m RNA expression between the control group and the severe dry eye group(P=0.000).Conclusion 1. 49.6% of the college students suffered from the low to moderate myopia. Only 15.4% of the college students suffered from high myopia. 2. The college students were in an unhealthy lifestyle such as stay up and reading for a long time. They often feel visual fatigue and dryness and most of them suffered from moderate dry eye. The prevalence of dry eye in the college students and in Chinese were semblable, and there was no statistical difference in different genders. That suggest there was a specific characteristic in college students who suffered from dry eye. 3. For the control group, there were no statistical difference in MUC1、MUC2、MUC4 、 MUC5 AC 、 MUC7 and MUC16 m RNA expression between males and females. 4. For the severe dry eye group, the MUC2、MUC4 and MUC5 AC m RNA expression in male were lower than the female. 5. For both the control group and the severe dry eye group, mucins m RNA expression were not all the same between males and females. But MUC5 AC m RNA expression in males and females seemed similarity and it decreased in severe dry eye group. 6. The results could speculate that the decreased m RNA expression of MUC2、MUC4 and MUC5 AC may influence the tear film stability. The relationship between the changes of MUC5 AC m RNA expression and the severity of dry eye more closely in the healthy young people.