Expression And Significance Of IL-17、NF-κB And CRP In Serum And Placenta Of Patients With Preeclampsia

Objectives:Measuring the expression of interleukin-17(IL-17)^ nuclear factor kB (NF-κB) andC reactive protein (CRP) in serum and placenta tissue of pregnant women with preeclampsia and normal pregnant women, to find the relationship between each other in preeclampsia.Explore the pathogenesis of preeclampsia and provide theoretical basis for the clinical diagnosis and treatment.Methods:Selecting 50 preeclampsia pregnancy women and 20 normal pregnant women.In 50 patients with preeclampsia, there are 20 patients with mild preeclampsia and 30 severe preeclampsia patients. Use ELISA and two step immunohistochemistry to detect the expression level of IL-17、NF-κB and CRP in serum and placenta tissue between the two groups. Excluding other complications of pregnancy and complications of internal medicine,there were no significant difference in the number of pregnancy,the number of delivery,the gestational weeks, age and body mass index (BMI) in the two groups. All pregnant women were collected before labor onset and all of them had the termination of pregnancy by cesarean section. Diagnostic basis is according to the Obstetrics and Gynecology (the Eighth Edition).Results:(1)The study found that preeclampsia placenta NF-κB expression level was significantly higher than that of the control group, significant differences (P< 0.05); severe preeclampsia placenta NF-κB expression level was significantly higher than that of mild preeclampsia group, significant differences (P< 0.05); and the expression levels of NF-κB in placenta of patients with mild preeclampsia were not significantly different than that in control group (P> 0.05).(2) Placenta tissue expression of IL-17 levels in Preeclampsia were significantly higher than those in the control group (P< 0.05); expression of IL-17 in severe preeclampsia placenta tissue was significantly higher than that of mild preeclampsia group (P< 0.05); the expression level of IL-17 in the placenta of patients with mild preeclampsia was not significantly different than that of the control group (P> 0.05).Through detecting the expression of IL-17 in serum find that the expression level of IL-17 in preeclampsia group was obviously higher than that of normal control group, and the difference is statistically significant (P< 0.05).(3) The expression level of CRP the placenta of preeclampsia was significantly higher than that in control group, the difference has statistical significance (P< 0.05), among them CRP in mild preeclampsia group was higher than that of control group, but the difference was not statistically significant (P> 0.05); severe preeclampsia group than in the control group and mild preeclampsia group increased obviously, the difference is statistically significant (P< 0.01). Through detecting the expression of CRP in serum find that the expression level of CRP in mild and severe preeclampsia group was obviously higher than that of normal control group, and the difference is statistically significant (P< 0.01).There were significant differences in the expression of CRP in the serum of severe preeclampsia group and mild preeclampsia group,but the difference was not statistically significant (P> 0.05).(4)Correlation analysis showed the expression of NF-κB, IL-17 and CRP in placenta tissues of preeclampsia have a positive correlation (P< 0.01) between each other. The expression level of IL-17 and CRP in the serum of patients with preeclampsia was positively correlated (P< 0.01).Conclusions:(1) The expression levels of NF-κB, IL-17, and CRP in the serum and placenta of preeclampsia patients were significantly higher than those in the normal control group. Besides, expression levels and the severity of preeclampsia have a significant relationship.(2) The expression levels of NF-κB, IL-17 and CRP in serum and placentas of preeclampsia women were positively related to the expression of the protein, which may be related to the pathogenesis of preeclampsia. It indicates that the inflammatory reaction is involved in the pathogenesis of preeclampsia.