Research Of Changes Of Serum MPO And Quantitative Study Of MPO MRNA Expression In Placenta Of Patients With Preeclampsia

ObjectiveTo investigate the relationships between concentrations of MPO level in serum andumbilical cord blood and MPO mRNA expression in placental tissue andpreeclampsia.Discusses the relationship between blood lipid metabolism and insulinresistance and oxidative stress in preeclampsia pathophysiological mechanism ofpossible role. And discusses the relationship between MPO level, high sensitivec-reactive protein (hs-CRP) of umbilical cord blood and and birth weight.MethodsA total of60women with PE and60normal pregnant women as control participatedin this study. Patients with PE were divided into early-onset group (n=30,presented at<34weeks of gestation), and lated-onset group (n=30,presented at≥34weeks ofgestation), with30normal pregnant women as early control group(<34weeks ofgestation) and30as late control group(≥34weeks of gestation).Enzyme linked immunosorbent (ELISA) method was used to detect serum MPO、insulin level; Application of automatic biochemical analyzer was used to test blood fatand blood sugar. The scattering turbidimetric method was used for determination of hs-CRP. Real time fluorescence quantitative PCR technology was used to detectplacenta tissue MPO mRNA expression level of the test-case groups and controlgroup2.Results1．serum MPO level of early and late-onset PE were higher than control group1andcontrol group2, difference was statistically significant (P <0.05); And significantdifference was found between early and late-onset PE(P <0.05), but no differencebetween the control group1and control group2(P﹥0.05).2．The mean serum levels of total cholesterol (TC)、triglyceride (TG)、low densitylipoprotein (LDL)、fasting insulin (FINS)、steady state model of insulinresistance index (HOMA-IR) were higher than contral group1and group2,while density lipoprotein (HDL) was lower than the control groups (P <0.05).difference was found in the comparisons of four groups (P <0.05); but nodifference between early and late-onset PE groups,or between the control group1and2(P﹥0.05).3．Serum hs-CRP level of early-onset and late-onset PE group were higher thancontrol group1and control group2, the differences were statistically significant(P<0.05); omparison of early-onset and late-onset PE group, the difference wasnot statistically significant (P﹥0.05).4．Placenta tissue MPO mRNA expression level of early-onset and late-onset PEgroup were higher than that of control group2, respectively, the differences werestatistically significant (P﹤0.05); no significantly difference was found betweenearly-onset and late-onset PE group (P﹤0.05).5．Umbilical cord blood MPO level of early-onset and late-onset PE group werehigher than those of control group2(P﹤0.05); the difference was statisticallysignificant between early-onset than late-onset group (P﹤0.05).6．Umbilical cord blood MPO level and placental tissue MPO mRNA expressionlevels of early-onset and late-onset PE group were positively correlated, thedifferences were statistically significant (P﹤0.05); while the control group withno obvious correlation, the difference was not statistically significant (P>0.05).7． MPO and serum TG, FINS, HOMA-IR level of early-onset and late-onset PEgroup were positively correlated (P﹤0.05), and the blood HDL was negativelycorrelated (P﹤0.05).8．Serum MPO and placental tissue MPO mRNA expression level of early-onset andlate-onset PE group were positively correlated (P <0.05). Control group with nosignificant correlation, the difference was not statistically significant (P>0.05).9．Umbilical cord blood MPO, hs-CRP level and neonatal birth weight of early-onsetand late-onset PE group were negatively correlated (P﹤0.05); while the controlgroup with no obvious correlation, the difference was not statistically significant(P>0.05). Conclusion:1．Serum, placental MPO expression levels of severe preeclampsia patientssuggesting that they may be involved in the pathophysiological process ofpreeclampsia.2．The placental tissue MPO mRNA expression level of severe preeclampsia patientswas significantly increased, and a positive correlation with serum and umbilicalcord blood MPO level, may rise MPO levels of serum and umbilical cord blood.3．Dyslipidemia and sugar metabolic abnormalities with severe preeclampsia patientsare concerned with serum MPO levels, they may be involved in the oxidativestress process of preeclampsia.