| ObjectivesResearch shows that 5-hydroxytryptamine (5-HT) is related with accident proneness of driver. This study was conducted to investigate the association between 5-hydroxytryptamine (5-HT) pathway related gene polymorphism and and level of related protein with accident proneness of driver, to explore the influence factors of drivers’accident proneness in genetic and biological way, explore the mechanism of how 5-HT affect drivers’accident proneness, aiming at providing a theoretical basis for reducing road traffic injuries.MethodsA category matching case-control study was conducted, drivers who had physical examination in Guangxi workers hospital in 2014 were brought into this study. According to their questionnaire surveys,300 drivers who had three or more accidents in the past 5 years were in the case group and 300 drivers who had no accident in the 5 years were selected as controls. The cases and controls were frequency matched in age, genders, vehicle types and driving years. High flux TaqMan MGB real-time fluorescent quantitative polymerase chain reaction technology was applied to gene typing for rs5443 of G protein beta-3 subunit (GNβ3), rs6265 of brain-derived neurotrophic factor (BDNF), rs6740584 of cAMP-responsive element-binding protein (CREB). The binary unconditioned Logistic regression model was used to analyze the association between 5-HT pathway related gene polymorphism and drivers’ accident proneness, as well as to evaluate the interaction between the SNPs and the environmental factors. Enzyme linked immuno sorbent assay (ELISA) was applied to determine the level of CREB in serum of 200 drivers (100 in the case group and 100 in controls), Statistical analysis was performed using t test and chi square test.Results(1) Distributions of rs5443 genotypes in the cases and the controls had statistical significance (χ2=7.20, P=0.027). Distributions of rs6265 and rs6740584 genotypes had no statistical significance between the cases and the controls.(2) The Logistic regression showed that the rs5443 TT genotype decreased the risk of drivers’accident proneness, polymorphism of rs6265 and rs6740584 had no statistical association with drivers’ accident proneness (rs6265:χ2=0.24, P＝0.888; rs6740584:χ2=0.01, P=0.990).(3) Interaction analysis indicated that polymorphism of rs5443 (OR=0.614, 95%CI:0.384-0.982, P=0.042), rs6265 and rs6740584 had no interaction with environmental factors (P>0.05).(4) Gene-environment interaction analysis found no interaction between this three loci and environmental risk factors.(5) The gene-gene interaction analysis found no interaction between this three loci.(6) The level of serum CREB had no statistical significance between the cases(3.21±1.90ng/ml) and controls (3.43±1.64ng/ml)(t=-0.892, P=0.374).(7) Stratification analysis on alcohol consumption and everyday sleep time showed that The level of serum CREB no statistical significance between the cases and controls.ConclusionGNβ3 rs5443 gene polymorphism may be associated with drivers’accident proneness, rs5443 TT genotype decreased the risk of drivers’accident proneness. BDNF rs6265 and CREB rs6740584 was not related to the accident proneness. The level of serum CREB was not related to the accident proneness. |
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