Association Between IL-1β-31T/C Polymorphism And Preeclampsia In Chinese Han Population

Objective : The purpose of our study was to investigate the relationship between the IL-1β-31T/C polymorphism and preeclampsia(PE).Methods:The genotyping for polymorphism at-31C/T of IL-1β was performed with the technology of Polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP) in 232 PE patients and 447 normal pregnant women.Demographic and clinical data were analyzed using Student’s t-test or Mann-Whitney test.The statistic differences of genotypic and allelic frequencies between case and control groups were compared by chi-square test.Results:The PCR fragments of the-31T/C polymorphism in the promoter region of IL-1βwere digested by Ava Ⅰ enzyme and then separated by electrophoresis on agarose gel,we got three different combinations of the electrophoretic bands: TT: one band of 239 bp,CC: two bands of 137 bp and 102 bp, CT: three bands of 239 bp, 137 bp and 102 bp. We then randomly chose the PCR fragments of several samples with three different genotypes(TT, CC and CT) and verified the accuracy of the results by sequencing, the results of which indicated that we were right. The genetic distributions of the-31T/C polymorphism in the promoter region of IL-1β in case and control groups were examined by Hardy-Weinberg equilibrium, the P >0.05 indicated that both cases and controls had a group representative. There existed an obvious statistic difference of the genotypic frequencies at-31C/T of IL-1β between cases and controls( X2=11.478, P=0.003).Pregnant women with the C allele had a higher risk for PE compared with those with T allele. The risk for women with CC genotype developing PE was 1.788 times of those without CC genotype.(X2=11.476, P=0.001, OR=1.788, 95%CI=1.275-2.506)Conclusion : The polymorphism of the promoter region-31T/C locus of IL-1 β is associated with genetic susceptibility to PE in Chinese Han population. The pregnant women with CC genotype had a higher risk for PE. The frequency of C allele in PE patients were greatly higher than normal pregnant women, and after the degree analysis of relative risk, we concluded that the C allele was one susceptible gene of PE. The risk for women with CC genotype developing PE was 1.788 times of those without CC. The aimof our present study was to look for new susceptible genes of PE and help clinical doctors to diagnose PE in genetic level, thus reduce occurrence of PE and guarantee the maternal and infant health.