A Study Of COMT And NQO1 Polymorphisms And Genetic Susceptibility To Preeclampsia

Objective Preeclampsia(PE)is a common serious complication in pregnant women,the oxidative imbalance of human body is closely related to the occurrence and development of the disease.The products expressed by gene COMT and NQO1 all participate in the oxidative stress process in vivo.The aim of this study was to investigate the relationship between genetic polymorphisms of COMT rs4680/NQO1 rs1800566 and genetic susceptibility of preeclampsia in Han women in Shandong province.Methods According to the diagnostic criteria of preeclampsia and the inclusion criteria of normal control,we collected 600 confirmed preeclampsia patients as case group and 800healthy pregnant women as control group from Shandong hospitals.We took 2~3 ml of anticoagulated venous blood from all candidates and extracted the DNA together.Using DNA extraction kit for DNA extraction,and placed in-20°C refrigerator for later use.The genotypes of COMT rs4680 and NQO1 rs1800566 in pregnant women and controls were detected by Taqman real-time fluorescence quantitative PCR.Statistical analysis was performed using SPSS software,and the genotyping of the control group was tested with the Hardy-Weinberg equilibrium.Comparison of general clinical data between case group and control group using independent sample t test.The chi-square test was used to compare the difference in distribution of genotypes and alleles between the two groups.p<0.05 means statistically significant.Results(1)The distribution of genotypes in the COMT rs4680 and NQO1 rs1800566 loci was tested for Hardy—Weinberg equilibrium.The results showed that the genotype distributions of the two control groups were all consistent with the H-W(p>0.05),indicating that the control group had a representative population.(2)The comparison of general clinical data between the case group and the control group showed that there was no significant difference between the two groups in age(p>0.05),while systolic blood pressure,diastolic blood pressure,gestational weeks of pregnancy,abortion frequency,neonatal weight were significantly different(p<0.05).(3)The genotype and allele analysis of case group and control group showed that the two loci had no significant difference in the distribution of genotype and allele(COMT rs4680,genotype:?~2=1.080,p=0.583,allele:?~2=0.687,p=0.407;NQO1 rs1800566,genotype:?~2=0.162,p=0.922,allele:?~2=0.000,p=1.000).(4)There was no significant difference between the mild/severe preeclampsia and the early-onset/late-onset preeclampsia compared with the control group(p>0.05).Conclusions(1)The results of this study show that the polymorphisms of COMT rs4680and NQO1 rs1800566 are not significantly associated with early/late preeclampsia in Shandong Han women.(2)There was no significant correlation between COMT rs4680and NQO1 rs1800566 gene polymorphisms and the incidence of mild/severe preeclampsia in Shandong Han women.(3)The polymorphisms of COMT rs4680 and NQO1 rs1800566 were not associated with preeclampsia in Shandong Han women.(4)Preeclampsia is affected by various factors such as environment and ethnicity.Therefore,we need to expand the scope of sample collection,increase the sample size,and further study the role of different genes in different ethnic groups.