The Study Between CYBA Polymorphisms In Shandong Han Women And Genetic Susceptibility Of Preeclampsia

Objective: To investigate the association of N icotinamide adenine dinucleotide phosphate(NADPH)oxidase p22 phox CYBA genes rs9932581 and rs1049255 polymorphisms in Shandong Han women with genetic susceptibility to preeclampsia(PE)to provide a new theoretical basis for the pathogenesis of PE.Methods: According to the diagnostic criteria of PE,we mainly collected the serum samples of pregnant women from the Qingdao University Affiliated Hospital,Qingdao Women’s and C hildren’s Hospital,Linyi people’s Hospital,Yantai Mountain Hospital,Jining Affiliated Hospital,Liaocheng People’s Hospital,Zibo Maternal and Child Health Hospital and so on,1029 patients were as PE group and 1400 normal pregnant women were as control group.The participants have no the serious organic disease and obstetric complications.We collected routinely the peripheral venous blood 2 m L from PE group and control group(EDTA anticoagulant),it was fully mixed and then taken 300 ul whole blood with 1.5 m L EP tube,and saved in the 4℃ refrigerator.The genomic DNA was extracted within 48 h and placed in the-20℃ refrigerator.The genotypic distribution of the rs9932581 and rs1049255 polymorphisms in CYBA were applied by Taqman Employing Q uantitative Real-time PC R;The group representativeness is tested by the Hardy-Weinberg genetic balance;The differences in general clinical data of the two groups were analyzed by the student’s t-test or nonparametric test.The difference of genotype and allele frequency distribution between the two groups was used to the Pearson-?2.The degree of relative risk was shown by the odds ratio and 95% confidence interval,there was significant statistical difference if the bilateral P-value less than 0.05.Results: The distribution of rs9932581 and rs1049255 polymorphisms in CYBA gene were in accordance with Hardy-Weinberg genetic equilibrium test in the control group(rs9932581: ?2 = 0.269,P = 0.604;rs1049255: ?2 = 0.198,P = 0.656),the experimental sample has the group representative.The comparison between the two groups in the demographic and clinical data showed there was no significant difference in gestational age,age of menarche,the number of pregnancy and frequency of abortion(p>0.05);However,there were significant differences in the gestational weeks,the blood pressure,white blood cells and neutrophil count(p < 0.05).The genotypic or allelic frequencies of the rs9932581 and rs1049255 were not different between the case group and control group(rs9932581,genotype χ2 = 1.444,P = 0.486 and allele χ2 = 0.164,P = 0.686,OR = 1.024,95%CI = 0.913-1.148;rs1049255,genotype χ2 = 4.637,P = 0.098 and allele χ2 = 0.686,P = 0.407,OR = 0.952,95%CI = 0.847-1.070);There were also no significant differences in genetic distributions between the mild /severe PE and the early/late-onset PE and control subgroups in the two polymorphism sites(p(29)0.05).Conclusions: The experimental results showed that the polymorphisms of rs9932581 and rs1049255 in CYBA are not associated with the development of the mild/severe PE and the early-onset/late-onset PE in Shandong Han women;And the genetic variants of rs9932581 and rs1049255 in CYBA might not be related to PE.However,the polymorphisms are still to be further verified in other populations.In order to explore the pathogenesis of PE from the perspective of function and genetics,expanding the number of samples,collecting multiple regions and exploring the role of different genes in a variety of population are needed to to discover the new PE susceptible genes and provide a basis for the early prevention,diagnosis and treatment of PE.