Association Between Polymorphisms Of PPARγ Genes And Susceptibility For Uygur’s Metabolic Syndrome

Objective:1.To investigate the distribution and linkage disequilibrium of peroxisome proliferator activated receptors γ gene 18 SNPs between MS group and control group in Uygur.2.To study the association of peroxisome proliferator activated receptors γ gene 18 SNPs with metabolic syndrome and their haplotypes with metabolic syndrome in Uygur.Methods:1. Randomized sampling 250 MS patients as MS group and 248 non-MS subjects for control group in Uygur from the data we collected before.2.Use a matrix-assisted laser desorption/ionization–time of flight to detect 498 subjects’ peroxisome proliferator activated receptors γ gene 18 SNPs.3.All the calculations and statistics were performed with the computer program, SPSS version 17.0.Statistical analysises adopt χ2 test, t test and one-way logistic regression, and calculate the values of odds ratio and 95% confidence intervals for odds ratio. Linkage disequilibrium and haplotype was analysed by SHEsis software.Results:1. After determination of polymorphisms of PPARγ, no significant differences in frequency distributions of three genotypes and allelotypes were noted between the MS group and the control group(P>0.05).2. The frequencies of CG genotype, GG genotype, G allele of rs1801282 for metabolic syndrome and controls in Uygur were(CG genotype: 26.8%vs24.0%, GG genotype: 0.8%vs3.7%,P=0.084; G allele:14.0%vs15.6%,P=0.556); The frequencies of CT genotype, TT genotype, T allele of rs3856806 for metabolic syndrome and controls in Uygur were(CT genotype: 36.0%vs32.4%,TT genotype:3.2%vs6.4%,P=0.197; T allele: 21.4%vs22.8%,P=0.597); The frequencies of TC genotype, TT genotype, T allele of rs4684847 for metabolic syndrome and controls in Uygur were(TC genotype: 26.0%vs22.8%,TT genotype: 0.8%vs4.8%,P=0.083;T allele: 13.8%vs15.2%,P=0.536);The frequencies of AG genotype, AA genotype, A allele of rs12490265 for metabolic syndrome and controls in Uygur were(AG genotype:35.2%vs37.2%, AA genotype: 6.8%vs9.6%,P=0.736; A allele: 24.4%vs26.4%,P=0.463).3.The levels of WHR in CT/TT genotype in Uygur of rs3856806 were significantly lower than those in CC genotype(P<0.05); The level of HC、TC、LDL-C、FPG、SBP and DBP in GA/AA genotype in Uygur of rs12490265 was significantly lower than those in GG genotype(all P<0.05); The levels of DBP in CG/GG genotype in Uygur of rs1801282 were significantly lower than those in CC genotype, WHR was higher than that in CC genotype(all P<0.05); The level of DBP and WHR in CC genotype in Uygur of rs4684847 was significantly lower than those in CT/TT genotype(P<0.05).4.Comparison of PPARγ genotype and allele distribution frequencies in MS group:After the risk analysis in the Uygur MS group, carriers with rs1801282 CG/GG genotype were0.552 times more possible than those with CC genotype to get abnormal blood pressure, compared with G allele, carriers with C allele were 1.746 times more likely to get abnormal blood pressure in subjects with MS(P<0.05).After the risk analysis in the Uygur MS group, carriers with rs4684847 CT/TT genotype were1.851 times more possible than those with CC genotype to get abnormal blood pressure, compared with T allele, carriers with C allele were 0.562 times more likely to get abnormal blood pressure in subjects with MS(P<0.05).After the risk analysis in the Uygur MS group, carriers with rs12490265 GA/AA genotype were0.567 times more possible than those with GG genotype to get abnormal blood pressure, compared with A allele, carriers with G allele were 0.596 times more likely to get abnormal blood pressure in subjects with MS(P<0.05).After the risk analysis in the Uygur MS group, carriers with rs3856806 CT/TT genotype were 0.264 times more possible than those with CCgenotype to get pathoglycemia in subjects with MS(P<0.05).5.Significant linkage disequilibrium was observed between peroxisome proliferator activated receptorsγ gene rs1801282 and rs4684847, rs3856806 and rs4684847, rs3856806 and rs1801282.6.Seven haplotypes were found between peroxisome proliferator activated receptors γ gene rs1801282、rs3856806 and rs4684847, and the frequencies of haplotype TGT was significantly different between MS and control group in Uygur(χ2TGT=5.914, P=0.023)..Conclusions :1.In 18 SNPs of peroxisome proliferator activated receptors γ gene may not associated with metabolic syndrome in Uygur.2. CG/GG genotype of rs1801282 had beneficial effect for blood pressure in Uygur;CT/TT genotype of rs3856806 had beneficial effect for obesity in Uygur; AG/AA genotype of rs12490265 had beneficial effect for blood pressure hyperlipemia and hyperglycaemia in Uygur; CC genotype of rs4684847 was associated with blood pressure and obesity in Uygur.3. CC genotype and C allele of rs1801282,CT/TT genotype and T allele of rs4684847,GG genotype and G allele of rs12490265 were risk factors for dysarteriotony in in subjects with MS. CC genotype of rs3856806 is a risk factor for pathoglycemia in in subjects with MS.4. There was a linkage disequilibrium between peroxisome proliferator activated receptors γ gene rs1801282 and rs4684847, rs3856806 and rs4684847,rs3856806 and rs1801282.5.The TGT haplotype maybe serve as protective factors of metabolic syndrome in seven haplotypes.