The Relationship Between Gene Polymorphism Of Adiponection Gene Promoter Region-11391Site And Metabolic Syndrome

Objective: To investigate the relationship and risk between metabolic syndrome (MS) and genepolymorphism of adiponection (APN) gene promoter region-11391site (G/A) by detecting the genepolymorphism of APN gene promoter region11391site.Method: A total of196patients with preliminary diagnosed as MS were selected as the casegroup, based on2004Chinese Diabetes Society diagnostic (CDS) criteria.213healthy subjects wereselected as the control group. The indicators of fasting plasma glucose (FPG), total cholesterol (TC),triglyceride (TG), HDL-C, FIN, BMI, systolic blood pressure (SBP), diastolic blood pressure (DBP) andnonprotein nitrogen (NPN) were measured, and serum APN expression was detect by Enzyme-linkedimmunoadsordent assay (ELISA). DNA was sampled using DNA extraction kit, then genotype and allelefrequencies of APN gene-11391site were identified by Polymerase chain reaction restriction fragmentlength polymorphism(PCR-RFLP). Risk between gene polymorphism of APN gene-11391site and MSwere evaluted by Logistic regression analysis.Resμlts(1) There was no statistical difference in age, gender, smoking and drinking in two groups (Age:t=1.546, P=0.123. Gender: χ~2=1.727, P=0.189. Smoking: χ~2=0.820, P=0.365. Drinking: χ~2=1.675, P=0.196).Genotype frequencies of control group comformed to Hardy-Weinberg equilibrium law, it is showed thestudy population groups were representative.(2) The gengtype (GG, GA and AA) frequenciesof APN gene-11391site were73.47%,18.88%and7.65%respectively in case group, and gengtype frequencies were88.73%,9.39%and1.88%respectively in control group, and mutation genotype frequency in case group was significantly higher thancontrol group. There were statistical differences in genotype frequency distribution in two groups (χ~2=9.479, P<0.001). The allele (G and A) frequencies of APN gene-11391site were82.91%and17.09%respectively in case group, and allele frequencies were93.43%and6.57%respectively in control group,and mutation allele frequency in case group was significantly higher than control group. There were statistical differences in allele frequency distribution in two groups (χ~2=22.006, P<0.05).(3) APN expression in case group was significantly lower than control group (t=7.459, P＜0.05).According to genotype classification of two groups, the indicators of APN, HDL-C and NPN in GA+AAgenotype group were significantly lower than GG genotype group (P＜0.05), and the indicators of FPG,FIN, TC, TG, BMI, SBP and DBP in GA+AA genotype group were significantly higher than GG genotypegroup (P＜0.05).(4) Compared with GG genotype carriers, the risk of GA genotype carriers suffering from MSincreased by1.083times (95%CI=1.016-1.114, P=0.025), and the risk of GA genotype carriers sufferingfrom MS increased by1.562times (95%CI=1.013-2.064, P=0.001).Conclusions: The gene polymorphism of APN gene promoter region-11391site is closelyrelated to the occurrence of MS, and genotypes of GA and AA were risk genotypes. The mutant geneaffects serum APN expression, thereby affecting a variety of matabolic and resulting abnormal chages ofbiochemical indicators. The risk of mutation genotypes of GA and AA carriers suffering from MSincreased.