Restrain Into Fat Differentiation Ability Of Bone Marrow Mesenchymal Stem Cells To Alleviate The Experimental Study Of Osteoporosis

Objective:The purpose of this study is to investigate the inhibitory effect on cell analysis of early osteoporosis mesenchymal stem differentiation capacity of bone marrow.Methods:40 female SD rats were selected in this study, the body weight of 200-250 g. The dorsal approach to establish a rat model of osteoporosis by ovariectomy.Randomly divided into 4 groups: sham operation group, model group, bone marrow mesenchymal stem cell therapy group, RNA interference of bone marrow mesenchymal stem cells in the treatment group;10% fetal bovine serum 7d injected into the tail vein of sham operation group after modeling n=10 LG-DMEM medium;10% fetal bovine serum 7d injected into the tail vein of n=10 model group after modeling LG-DMEM medium;Bone marrow mesenchymal stem cells of bone marrow mesenchymal stem cells in the treatment group 7d intravenous injection of n=10 after modeling;RNA interference, RNA interference of bone marrow mesenchymal stem cells of bone marrow mesenchymal stem cells in the treatment group7 d intravenous injection of n=10 after modeling.To the end of the intervention, in accordance with the measurement of bone density on the packet, RNA interference of bone marrow mesenchymal stem cells were evaluated in the early curative effect of osteoporosis;Comparison of four groups of rats blood biochemical markers of bone turnover osteoporosis early bone formation and bone resorption changes.Results:Bone density detection: The bone density of RNAi transfected BMSCs in four groups was higher than model groups differences was statistically significant(P<0.05);The bone mineral density in BMSCs treatment group was higher than model groups differences was statistically significant(P<0.05);The bone was RNAi transfected BMSCs was significantly higher than that BMSCs treatment groups differences was statistically significant(P<0.05);The bone was RNAi treated group and BMSCs treated group was lower than operation groups differences was statistically significant(P<0.05).Four groups of rats RNAi transfected BMSCs treatment groups PINP higher than the model groups differences was statistically significant(P<0.05);PINP was significantly higher than model groups and treatment group differences were statistically significant(P<0.05);RNAi transfected BMSCs treatment group PINP is higher than the BMSCs treatment groups differences was statistically significant(P<0.05);show that the two groups in osteoporosis early RNAi transfected BMSCs treatment group PINP is better than that of the BMSCs treatment group.RNAi was transfected with BMSCs and PINP was lower than that of sham operation group in treatment group and BMSCs differences was statistically significant(P<0.05).Four groups of rats RNAi transfected BMSCs treatment group beta CTX lower than the model groups differences was statistically significant(P<0.05);BMSCs treatment group beta- CTX was significantly lower than the model group, with statistical significance(P<0.05);RNAi transfected BMSCs treatment group A beta- CTX below the BMSCs treatment group, significant difference, with statistical significance(P < 0.05), show that the two groups in osteoporosis early RNAi transfected BMSCs treatment group A beta-CTX than BMSCs treatment group.The RNAi treated group and BMSCs treated group and BMSCs treated group beta- CTX were higher than those in sham operation group(P >0.05).Conclusions:RNAi transfected with BMSCs content improve bone in ovariectomized rats, promote bone formation.