The Study Of The Expression Of MAPK In Basal Cell Carcinoma And Bowen’s Disease

Objective To explore the role of ERK1/2(extracellular signal-regulated kinase1/2), JNK(c-Jun N-terminal kinase) and P38MAPK(P38 mitogen-activated protein kinase) in the occurrence and development of basal cell carcinoma(BCC) and Bowen’s disease by detecting the expression of the activated form of the three major members of MAPK(mitogen-activated protein kinase): ERK1/2, JNK and P38 MAPK, namely the phospho-ERK1/2(p-ERK1/2), phospho-JNK(p-JNK) and phospho-P38MAPK(p-P38MAPK).Methods Collecting 33 specimens of basal cell carcinoma, 17 specimens of Bowen’s disease, which were all diagnosed by Pathology, and 17 specimens of normal skin tissues. Immunohistochemistry and Western blot techniques were used to detect the expression of p-ERK1/2, p-JNK and p-P38 MAPK in basal cell carcinoma, Bowen’s disease and normal skin. And to detect their interrelationship in basal cell carcinoma and Bowen’s disease.Result(1) The expression of the phospho-ERK1/2 in Immunohistochemical technique: The AOD values in basal cell carcinoma and Bowen’s disease were all higher than normal skin, the difference was statistically significant(P<0.05); The AOD value in basal cell carcinoma was significantly higher than Bowen’s disease(P<0.05).(2) The expression of the phospho-ERK1/2 in Western blot: In basal cell carcinoma and Bowen’s disease, the expression of phospho-ERK1/2 were higher than normal skin(P<0.05); Basal cell carcinoma protein content was also higher than Bowen’s disease(P<0.05).(3) The expression of the phospho-JNK in Immunohistochemical technique: The AOD values in basal cell carcinoma and Bowen’s disease were all higher than normal skin, the difference was statistically significant(P<0.05); The AOD value in basal cell carcinoma was significantly higher than Bowen’s disease(P<0.05).(4) The expression of the phospho-JNK in Western blot: In basal cell carcinoma and Bowen’s disease, the expression of phospho-JNK were higher than normal skin(P<0.05); Basal cell carcinoma protein content was also higher than Bowen’s disease(P<0.05).(5) The expression of the phospho-P38 MAPK in Immunohistochemical technique: The AOD values in basal cell carcinoma and Bowen’s disease were all higher than normal skin, the difference was statistically significant(P<0.05); The AOD value in basal cell carcinoma was significantly higher than Bowen’s disease(P<0.05).(6) The expression of the phospho-P38 MAPK in Western blot: In basal cell carcinoma and Bowen’s disease, the expression of phospho-P38 MAPK were higher than normal skin(P<0.05); Basal cell carcinoma protein content was also higher than Bowen’s disease(P<0.05).(7) The relation of phospho-ERK1/2 and phospho-JNK was not correlated in basal cell carcinoma and Bowen’s disease(coefficient of Spearman’s correlation was 0.785, P=0.064 and 0.183, P=0.728). The relation of phospho-ERK1/2 and phospho-P38 MAPK was not correlated in basal cell carcinoma and Bowen’s disease(coefficient of Spearman’s correlation was-0.743, P=0.091 and 0.535, P=0.275). The relation of phospho-JNK and phospho-P38 MAPK was not correlated in basal cell carcinoma and Bowen’s disease(coefficient of Spearman’s correlation was-0.604, P=0.204 and-0.206, P=0.696).Conclusions(1) ERK1/2, JNK and P38 MAPK played an important role in the occurrence and development of basal cell carcinoma and Bowen’s disease.(2) The expression levels of phospho-ERK1/2/JNK/P38 MAPK were associated with the malignant degree of tumors.(3) The activation of the three major members of MAPK were alone and independently between each other.