Association Between Interleukin23R Gene Polymorphisms And Oral Lichen Planus

Oral lichen planus（OLP） is a common oral mucosal disease，chronic deferment or relapse, difficult to cure. It is composed of Tcell-mediated chronic inflammatory diseases, CD4+T lymphocyteimmune abnormalities play a key role in the pathogenesis. It’spathological features are: epithelial dysfunction keratosis, liquefactiondegeneration of the basal layer and dense lymphocytic infiltration zone inthe lamina propria.IL-23is a cytokine of inflammatory disease,autoimmune diseasesand cancer. As the IL-23signal transfer factor, IL-23R is one of the twosubunits of the complex.IL-23R has gradually attracted people’s attention,since it was first reported in2002. Related literatures have been reportedthat IL-23R is important in the pathogenesis of inflammatory diseases,autoimmune disease and malignant tumor susceptibility, but the researchin oral squamous lichen is close to no.ObjectiveTo investigate single nucleotide polymorphisms（SNP）of interleukin23R gene with oral lichen planus（OLP） in China, and to provide animportant genetic basis in exploring the disease susceptibility and diverse clinical manifestation.Methods66patients with OLP (21erosive OLP patients) and70healthycontrol subjects were recruited. Genomic DNA was extracted and PCRproducts spanning two SNPs of the IL-23R gene at positions（rs10889677and rs11465817） were analyzed. The genotype frequencies werecalculated and analyzed.Results:1. In this experiment both of the two sites (rs10889677,rs11465817)of IL-23R had been detected three genotypes AAhomozygote, AG heterozygote, GG homozygote.2. The rs10889677, rs11465817allele and genotype frequencydistribution of the difference in the case group and the control group hadno statistical significance (P>0.05).3. The rs10889677genotype frequency distribution in OLPcompared with the control group had significant difference (P=0.024), theCC+CA genotype of the individual risk occurrence of erosion type is3.115times of the AA genotype (95%CI:1.135~8.548).Conclusion:1. Both sites (rs10889677, rs11465817) in IL-23R gene of the OLPpopulation have genetic polymorphism.2. The polymorphism of rs11465817locus had no significant correlation existed in OLP disease susceptibility;3. The polymorphism of rs10889677point mutation may beassociated with disease susceptibility and severity of OLP.