| Objective:According to the principles of traditional Chinese medicine as supplementing qi, nourishing Yin, promoting blood circulation, GanCaoSanShen Tang(GCSST) is designed on the basis of traditional Chinese medicine compound prescription " Zhigancao Decoction" and "Sheng Mai San", which is mainly used in clinical practice with obvious effect for the treatment of arrhythmia caused by deficiency of heart qi, blood stasis, heart meridian impassability. The previous study showed that it has a protective effect on arrhythmia, and can improve the state of myocardial ischemia in mice, ability of anti-fatigue, function of accelerating blood circulation, which provided a preliminary experimental basis to research and development of the prescription. Heart and cerebrovascular diseases associated with microcirculation disorder is one of the most common diseases, so the researches on microcirculation are of profound significance. In order to explore the affects of GCSST on cardiovascular disease, especially the direct affects on microcirculation, In the first part of this paper, the relationships between Microcirculation disturbance and TCM collateral disease, syndrome of blood stasis were discussed and summarized based on the profound analysis and contrastive study of existing references. In the second part, the influences of Chinese medicine compound GCSST on microcirculation of mice auricle, mesenteric blood microcirculation and blood rheology were verified through the experiments, on the basis of which, effect and mechanism of the GCSST to improve blood microcirculation were investigated. which provide the experimental basis for the further development research and clinical application of the prescription. Methods:Experiments investigate influence of GCSST on blood microcirculation of test mice. The experimental animals were randomly divided into five groups of 10, namely saline control group, aspirin positive control group, GCSST with large, medium and small dose group. Continuous gavages had been done for six days, and after the last dosing, micrangiums in the region of stable flow were selected and marked, and animal model of microcirculatory disturbance was established through dripping 1:1000 epinephrine into the observation area of test mice. Then diameter and blood flow velocity of auricle and mesenteric microvascular of the marking region before and after making model were observed and analyzed. The data of each group was analyzed by application of SPSS20.0 statistic software. Single factor analysis of variance was used to analyze statistical significance, and statistical significance of the discrepancies among different groups was analyzed through the LSD method. To be specific, experimental data were expressed as mean ± standard deviation( x ±s), in which P<0.05 indicates that the discrepancy was statistically significant and P<0.01 indicates that there are statistically significances.Experiments investigate influences of GCSST on hemorheology of test mice.The experimental animals were randomly divided into five groups of 10, namely saline control group, aspirin positive control group, GCSST with large, medium and small dose group. Continuous gavages had been done for six days, and after the last dosing, tail cutting method was applied to measure the bleeding time and slide method was used to record clotting time; eyeballs of the test mice were removed to collect blood, full automatic blood rheometer was applied to detect the whole blood viscosity(low and high shear rate), plasma viscosity and hemorheological parameters of HCT. The data of each group was analyzed by application of SPSS20.0 statistic software. Single factor analysis of variance was used to analyze statistical significance, and statistical significance of the discrepancies among different groups was analyzed through the LSD method. To be specific, experimental data were expressed as mean ± standard deviation( x ±s), in which P<0.05 indicates that the discrepancy was statistically significant and P<0.01 indicates that there are statistically significances. Results:1 Experiments investigate influences of GCSST on hemorheology of test mice1.1 as to the influences of microcirculation in the auricle, 15 min after modeling, results can be seen as follow: 1) the difference between microvascular diameter as well as flow velocity of the ear auricle of the mice and 5 groups before modeling is significant(p<0.01); 2) the differences between microvascular diameters of aspirin group as well as GCSST groups(large, middle and small dose) and the saline group are of statistic significance(p<0.05, p<0.01);3)there are obvious discrepancies(p<0.05)of blood flow velocity for GCSST(high and medium dose) and aspirin control groups compared with the saline group. It can be found that the microvascular diameter and blood flow velocity in saline group are of no significant change(P > 0.05)between modeling after 30 min and 15 min, meanwhile, the ones of aspirin group and GCSST group increased significantly(p<0.01).1.2 In the process of impacts on the mesenteric microcirculation, mesenteric microvascular diameter is significantly narrowed after the mesentery modeling for five groups of test mice(P<0.01). GCSST experimental group and the control group with aspirin are more significantly different from saline group(p <0.05); 2mins after modeling, flow velocities of blood in the auricle are much slower than this group before modeling(P<0.01); Similarly, GCSST with large, medium, small dose group and the control group with aspirin are more significantly different from saline group(p<0.05);blood flow velocity of saline group and GSCCST small dose group are of no significant difference, when compared modeling of mesentery microvascular after 8mins and 2mins. GCSST medium and large dose group as well as aspirin group increased obviously(p<0.05)2 Experiments of GCSST affect blood rheology of test mice.2.1 GCSST affects clotting time of test mice. Positive control aspirin group can significantly prolong clotting and bleeding time of mice relative to the saline group(p<0.01). all of the GCSST doses(large, medium and small) can prolong clotting and bleeding time, and compared with the control saline group, the discrepancy is significant(p<0.01, p<0.05); as to the influence on the clotting time, There are significant differences of small and medium dose of GCSST groups compared with the aspirin group(p<0.01), but the large dose group are in no statistical sense(p>0.05); it can be seen from the influence on clotting time that all doses of GCSST groups can prolong the clotting time compared with the control aspirin group, and the discrepancy is of the statistical significance(p<0.01).2.2 GCSST affects blood rheology of test mice. Compared with the saline group, positive control aspirin group can significantly reduce whole blood shear viscosity(both high and low), plasma viscosity and hematocrit. As the same of aspirin group, GCSST large, medium dose groups can also reduce the correlated indexes, and the discrepancy is of the statistical significance(p<0.01) compared with the blank group, however, the results of small dose group of GCSST is in no statistical sense(p>0.05). Conclusion1 Large, medium and small dose of GCSST after administration can inhibit the diameter reduction induced by epinephrine of microvascular diameter, which can also inhibit the descent of blood flow velocity. Meanwhile, after administration of GCSST, recovery of diameters of microvascular diameter as well as blood flow velocity can be promoted. Thereby the effects of increasing tissue blood perfusion, promoting microcirculation elimination and functional recovery can be achieved, and microcirculation function can be improved finally.2 GCSST can prolong clotting and bleeding time, and improve blood viscosity of microcirculation, which illustrates that GCSST has the effect of promoting blood circulation to improve the blood stasis symptom.3 GCSST can reduce high and low shear rate of whole blood viscosity, and reduce hematocrit as well as plasma viscosity significantly, which illustrates that GCSST can inhibit coagulation of erythrocyte aggregation, reduction of blood viscosity and concentration, Thereby, improve blood stasis intravascular and help to improve blood circulation.4 The effects of GCSST on improving microcirculation disturbance are multifaceted, the reason for which may be related to promoting blood circulation and removing blood stasis, invigorating qi and nourishing Yin of GCSST and the complexity of traditional Chinese medicine compositions. So the effect mechanism of GCSST needs further study. |
PDF Full Text Request