The Study About The Expression Of Beta-catenin Protein In The Cervical Intervertebral Disc Of Patients With Cervical Intervertebral Disc Herniation

Background Cervical intervertebral disc herniation is the result of aneck injury or chronic injury, but the injury eventually induce nervedysfunction based on the basis of degenerative changes of ervicaldegenerative.The diagnosis of cervical intervertebral disc herniation is basedmainly on abnormal MRI imaging and clinical performance while X-ray andcomputed tomography (CT) as an auxiliary examination. Its pathogenesis is notyet clear, studies have shown that a variety of cytokines and signaling pathwayswithin the intervertebral disc cells maintain environmental stability and thebalance of the intervertebral disc injury and repair with interaction, mutualcoordination and restraining each othe r.The stability and balance once havebeen broken, intervertebral disc tissue degeneration will occur. The Wnt/β-catenin signal transduction pathway become research focus in the role ofdegeneration of intervertebral disc. But we did not find out the experimentalreport about the detection ofβ-catenin protein in cervical intervertebral disc ofcervical intervertebral disc herniation patients.Objective The application of immunohistochemical method to detectβ-catenin protein in cervical intervertebral disc of cervical intervertebral discherniation patients and normal people, so as to explore its pathogenesis. Methods Collect16cases of surgery resection of disc tissue samples ofpatients with cervical intervertebral disc herniation as experimental group(13male and3female)，mean age45years (19-72years);5cases of normal disctissue samples of paients with the lumber spinal trauma surgery in the sameperiod as control group (3male and2female), mean age25years (18-35years).All the patients were surgical operated in our hospital from May2014to May2015. The expression of Beta-catenin protein were detected byimmunohistochemical method。Results Beta-catenin protein has significant expression in thedegeneration of cervical intervertebral disc tissue. However, it has noexpression in the control group of normal cervical intervertebral disc tissue.Conclusion Significant Beta-catenin expression in degeneration ofcervical intervertebral disc tissue prove Wnt/beta-catenin signaling pathwaymay play a role in the process of cervical intervertebral disc degeneration.