Effect Of Oral Probiotic Joint Trace Feeding On Complications And Immunity Of Premature Infants

ObjectiveWith the development of among medical and neonatal rescue techniques are widely used, especially in NICU, set up to provides more opportunities and conditions for premature babies. Premature babies is a special group, the digestive system and immune system function development is not mature, and more after caesarean section, unable to breastfeed, and need to use antibiotics, engraftment of time delay of intestinal normal flora, lack of intestinal bacterial species, especially the lack of bifidobacterium and lactobacillus. And the intestinal normal flora plays an important role in maintaining human health, lactobacillus and bifidobacterium is considered to be one of the most important human host of beneficial bacteria. Studies show that probiotics can make the establishment of the premature intestinal normal flora in advance, improve digestive and immune function in preterm neonates. In laiwu city maternal and child health care of 80 cases of premature infants as the research object, from the aspects of laboratory test and clinical observation to study the early oral probiotics joint trace feeding for preterm infant feeding intolerance, premature infant necrotizing enterocolitis(NEC), and other common complications and the effect of immunefunction. MethodologySelection of laiwu city maternal and child health care of neonatal intensive care unit(NICU) 80 cases of hospitalized premature infants were randomly divided into treatment group(40 cases) and control group(40 cases), two groups of premature infants were performed after admission warmers to keep warm, treating the primary disease, early trace feeding(6-12 h after admission to open milk diluted with 1/2 of trace feeding, low birth weight formula milk from 0.5 to 4.0 ml/kg/d, 1 time every 2-3 hours, according to the situation of children with tolerance to increase milk supply appropriately, without feeding intolerance, adding in 0.5ml/kg, and gradually increase concentration, gradually within 3-7 d no dilution of low birth weight formula feeding, until the daily milk increased to 80-100ml/kg/d, can’t give Bi Si lactation.), application of antibiotics to prevent infection and symptomatic support treatment, on this basis, the treatment group after open milk given bifidobacterium triple viable enteric capsules orally, 105 mg each time, twice a day, oral. 14 days. Observation in the hospital two groups of children with vomiting, abdominal distension and gastric retention during the feeding intolerance; Observe the incidence rate of two groups of children with NEC and severity; Respectively on admission and 14 days after extraction of peripheral venous blood 3 ml, using flow cytometry to detect the percentage of T lymphocyte subsets, fill with immune turbidity method to detect the concentration of two groups of immunoglobulin and complement.Statistical methods: statistical analysis using SPSS17 data analysis software, measurement data with mean(x±S)standard deviation said, two groups of continuous variable compared with t test, compare with X2 test for count data, to P <0.05 for statistical significance. Results1.Feeding intolerance: treatment group in vomiting, abdominal distension and gastric retention, a total of 4 cases, vomiting, abdominal distension and gastric retention and the control group a total of 12 cases, it is obvious that the treatment group in the proportion of feeding intolerance is far lower than the control group, the difference was statistically significant(P<0.05).2.NEC: NEC a total of 6 cases of control group, the Bell, class I l example, Bell class II(3 cases), Bell level III in 2 cases; Treatment group NEC has occurred in 1 cases,only the bell,class II in 1 case. The treatment group the incidence of NEC and severity is far lower than the control group, the difference was statistically significant(P<0.05).3.The T lymphocyte subgroup: two groups before treatment of premature peripheral blood T lymphocyte subgroup differences no statistical significance(P>0.05), oral probiotics 14 days after peripheral blood T lymphocyte subsets CD4+, CD4 + / CD8+ ratio level of treatment group is higher than the control group, the difference was statistically significant(P<0.05), the percentage of CD3+and CD8+ level change is not obvious, no statistical significance(P>0.05).4.Immunoglobulin and complement: two groups of premature infants before treatment differences in peripheral blood immunoglobulin and complement has no statistical significance(P>0.05), 14 days after the control immunoglobulin oral probiotics Ig M is lower than the treatment group, the difference was statistically significant(P<0.05), and immunoglobulin Ig G、C3 and C4 level change is not obvious, no statistical significance(P >0.05). Conclusion1.Oral probiotics joint early trace feeding can promote gastrointestinal peristalsis, promote gastric emptying, reduce the occurrence of feeding intolerance.2.Oral probiotics joint early trace feeding can promote the establishment of the intestinal flora, to improve the function of intestinal mucosa, reduce the incidence of NEC and severity.3.Oral probiotics joint early trace feeding can improve oral probiotics peripheral blood related immune index, promote the B cell mediated cells of the humoral immunity and T cell-mediated immunity, enhance the immunity of the premature infants.