| Objective: In order to ensure the safety and health of premature infants and provide a better way of to feed and manage them,a vitro fermentation model is used to analyse the glycolysis,acid production and gas production of oligosaccharides in the intestinal flora with different gestational ages' fresh fecal specimens.Materials and Methods: 30 preterm infants of different gestational ages(21 males and 9 females)enrolled in Shanghai Children's Hospital from July to December 2018 were selected and all of their gestational ages are ranged from 29 weeks to 36 weeks.According to the gestational age of the child,there were two groups,one of which is less than 32-weeks and the other is more than 32-weeks,acoording to different feeding pattern,it is divided into parent-child breast milk group,donated breast milk group and formula milk group.They were admitted to the neonatal ward of our hospital after birth and met following criteria:(1)All intestinal/near-intestinal feeding(150ml/kg.d)after admission;(2)The birth weight has been restored;(3)Antibiotics are discontinued;(4)There is no obvious feeding intolerance such as vomiting,abdominal distension,and gastric retention.And those infants were without congenital malformation severe heart,liver and kidney dysfunction and congenital inheritance Metabolic and chromosomal disorders.The fecal specimens were collected and treated,and then inoculated in 4 different culture media for 24 hours.After that,the contents of various short-chain fatty acids(SCFA)and 24 h gas production in feces were measured.Finally,those data were analysed by using software spss 25.0.Results:1.In 10% fecal diluent and medium LAT,FOS,GOS fermentation broth of two premature infants groups,the acid content has the similar distribution and those acid percent from high to low is acetic acid,butyric acid,propionic acid,and isobutyric acid,isovaleric acid and valeric acid.2.There was no significant difference in the content of acetic acid,propionic acid,butyric acid and air pressure between the preterm infants less than 33 weeks and the preterm infants more than 32-weeks.(P > 0.05)3.The feces of the premature infants in both groups can produce more butyric acid after fermentation in the medium GOS.4.The production of oligosaccharide medium in the preterm infants less than 32 weeks and in the preterm infants older than 32 weeks was significantly higher than that in the basal medium(P<0.05)5.The total acid content in the parental group was higher than that of the donor group which is bigger than that of the formula group and those groups were statistically significant because the P value is smaller than 0.05.There was a similar value of butyric acid content in the parent-child breast group and the donated breast milk group that is larger than that of the formula group.Conclusions:1.The oligosaccharides can be used for premature infants to produce more SCFA,as well as a large amount of gas.2.When the preterm infants of different gestational ages reach the whole intestinal feeding and correct the gestational age of 34 weeks,the ability of the intestinal flora to produce SCFA using oligosaccharides is similar.3.Intestinal flora of premature infants can use lactose to produce more beneficial SCFA and use GOS to produce more butyric acid.4.The premature infant who was feeded with parent-child breask milk can produce more SCFA,next is donated breask milk,the last is formula. |
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