Experimental Study Of Treating Acute Soft Tissue Injury By Partial Combined Acupoint Applicator On Rat Model

Objective:This study is going to explore the therapy effects and mechanism of treatment in acute soft tissue injury on rat model by partial combined acupoint applicator, and provide theoretical support of the therapy to clinical.Method:40 SPF SD rats (50% male and 50% female) were randomly divided into four groups (control group, model group, acupuncture combined partial application groups, partial application group). All groups were builded the model of acute soft tissue injury by free-fall hit method except the control group. The next day after modeling, acupoint combined partial application group was treated with acupoint combined partial application by Safflower, Panax, and Corydalis. The acupoints treated were ST36 and SJ5. The partial application group was treated by partial applicator only. The treatment would last for 5 day, once a day. The control group and model group were only General feeding. The condition and activity evaluation was taken at 1st,3rd, and 5th day. At the final day, the muscle tissue of injury part was collected from rat and made to homogenates. Then the IL-10 was tested in the homogenates by ELISA.Results:Before treatment, the model group, the results of condition and activity evaluation of acupoint combined partial application group and partial applicator group were significantly higher than control group (P<0.05). The result of condition and activity evaluation of model group was not statistically significant(P> 0.05) at before and after treatment. The evaluation results of acupoint combined partial application group and partial applicator group were significantly lower (P<0.05) than 1st day since 3rd day. Between the two groups, acupoint combined partial application group improved significantly more than partial applicator group (P<0.05). In the results of IL-1β, model group was significantly higher (P<0.05) than control group. And acupoint combined partial application group and partial applicator group were significantly lower (P<0.05) than model group. However, acupoint combined partial application group was significantly lower (P<0.05) than partial applicator group.Conclusion:Acupoint combined partial application could enhance the therapy effect in soft tissue injury on rats model with acute pain, limping, swelling. What is more, one of the mechanisms may be reducing IL-1β content to treat injury.